维生素E对果蝇辐射氧化损伤机制的影响

目的 探索不同浓度维生素E对γ射线照射后果蝇幼虫敏感性的影响。 方法 使用不同浓度（100、500、1000、1500 mg/L）维生素E处理W1118果蝇幼虫，137Cs γ射线 50 Gy 进行照射，测定果蝇幼虫的蛹化率和羽化率、羽化后48 h死亡率、过氧化氢酶（CAT）活性和谷胱甘肽（GSH）含量，分析其抗氧化能力和氧化损伤状态。组间的比较采用LSD-t检验。 结果 1000、1500 mg/L维生素E组果蝇幼虫的蛹化率、羽化率、攀爬能力、CAT活性、GSH含量均明显比单纯照射对照组高，且差异均有统计学意义（t=2.864~16.462，均P<0.05）。与未照射对照组比较，1000 mg/L和1500 mg/L维生素E组的果蝇幼虫羽化为成虫48 h内的死亡率分别从（54.0±5.0）%降低至（36.1±7.6）%和（37.5±5.8）%，差异均有统计学意义（t=3.386，P=0.028；t=3.718，P=0.021）。 结论 维生素E通过减少氧化性应激来减缓果蝇幼虫辐照后的氧化损伤，并能提高果蝇的辐射抗性。

Effects of vitamin E on oxidative damage mechanism of gamma radiation in Drosophila melanogaster

Objective To explore the effects of different concentrations of vitamin E on the sensitivity of fruit fly larvae after gamma-ray irradiation. Methods Different concentrations(100, 500, 1000, and 1500 mg/L) of vitamin E were used to treat W1118 fruit fly larvae, and 137Cs gamma-ray irradiation of 50 Gy was utilized. The pupation and eclosion rates, mortality rate after eclosion for 48 h, catalase activity(CAT), and glutathione(GSH) content of fruit fly larvae were measured, and the antioxidant capacity and oxidative damage status were analyzed. LSD t-test was used to compare groups. Results The pupation rate, emergence rate, climbing ability, CAT activity, and GSH content of fruit fly larvae in the vitamin E(1000 mg/L and 1500 mg/L) group were significantly higher than those in the control group, and the differences were statistically significant(t=2.864–16.462, all P<0.05). In comparison with the non-irradiated control group, the mortality rate of fruit fly larvae in the vitamin E group with concentrations of 1000 mg/L and 1500 mg/L significantly decreased from(54.0±5.0)% to(36.1±7.6)% and(37.5±5.8)%, respectively, within 48 h of emergence to adult(t=3.386, P=0.028; t=3.718, P=0.021). Conclusion Vitamin E can slow down the oxidative damage of Drosophila melanogaster larvae after irradiation by reducing oxidative stress, as well as improve the radiation resistance of Drosophila melanogaster.