乌索酸体外抑制人胃癌BGC-823细胞增殖的作用

目的探讨乌索酸(UA)对体外培养的人胃癌细胞系BGC-823增殖的抑制作用。方法用MTT法在不同浓度、不同给药时间、不同溶剂溶解的条件下,以5-Fu作对照,观察UA对人胃癌细胞系BGC-823细胞的体外增殖抑制效果。结果UA在低浓度条件下(5~20mg/L)对BGC-823细胞抑制率呈浓度依赖性。25~2000 mg/L时随着浓度的增加抑制率无明显差异;UA对BGC-823细胞作用呈时间依赖性;不同溶剂溶解UA,抑制率无差异(P>0.05);UA对BGC-823细胞抑制率明显高于5-Fu(P<0.01)。结论UA对体外培养的人胃癌细胞系BGC-823有明显的增殖抑制作用,用药量小时呈现浓度依赖性及时间依赖性;其增殖抑制作用强于5-Fu。溶剂不同对UA增殖抑制作用无影响。

Inhibitory effect of Ursolic acid on the proliferation of human gastric cancer cell line BGC-823 in vitro

Objective It is to explore the inhibitory effect of Ursolic acid(UA) on the proliferation of human gastric cancer cell line BGC-823 in vitro.Methods MTT assay was used at different concentrations,time phases and solvents of Urosolic acid and 5-Fu was used as control.Results Under the condition of lower concentration of UA(5-20 mg/L),the inhibitory rate(IR) of BGC-823 showed concentration dependent.Under the higher concentration(25-2?000 mg/L),there was no significant difference as the concentration increased.The effect of UA on BGC-823 cell showed time dependence(P>0.05).There was no difference in IR when treated with different solvents.Compared with 5-Fu,IR of BGC-823 by UA was obviously higher(P<0.01).Conclusion UA has obvious effect of proliferation inhibition on human gastric cancer cell line BGC-823.At lower concentration it has concentration and time dependence,and its inhibitory effect is better than that of 5-Fu.There was no difference in inhibitory effect on BGC-823 among different solvents.