| HPLC 法测定贯叶金丝桃中芦丁和金丝桃苷的含量 |
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| 引用本文： | 陈英红,董芳,姜翔之,姜瑞芝,王颖.HPLC 法测定贯叶金丝桃中芦丁和金丝桃苷的含量[J].中国药师,2012(10):1429-1430. |
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| 作者姓名： | 陈英红 董芳 姜翔之 姜瑞芝 王颖 |
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| 作者单位： | 吉林省中医药科学院;吉林大学中日联谊医院 |
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| 基金项目： | 吉林省科技发展计划项目(编号:20115092) |
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| 摘 要： | 目的：建立贯叶金丝桃中芦丁与金丝桃苷的含量测定方法。方法：采用高效液相色谱仪,使用Diamonsil C18柱（250mm×4.6 mm,5μm）,流动相为甲醇-乙腈-四氢呋喃-0.4%醋酸（1∶1∶18∶80）,流速为0.7 ml·min-1,检测波长为358 nm,柱温为30℃。结果：芦丁在0.206～1.236μg范围内有良好的线性关系,r=0.999 9。金丝桃苷在0.144～0.864μg范围内有良好的线性关系,r=0.999 8;芦丁的平均加样回收率为98.57%,RSD为0.94%,金丝桃苷的平均加样回收率为98.57%,RSD为0.94%。结论：该方法简便快捷,准确度高。
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| 关 键 词： | 贯叶金丝桃 芦丁 金丝桃苷 高效液相色谱法 |
| 收稿时间： | 6/9/2012 12:00:00 AM |
| 修稿时间： | 2012/7/10 0:00:00 |
Determination of Rutin and Hyperoside in Hypericum Perforatum L. by HPLC |
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| Chen Yinghong,Dong Fang,Jiang Xiangzhi,Jiang Ruizhi and Wang Ying.Determination of Rutin and Hyperoside in Hypericum Perforatum L. by HPLC[J].China Pharmacist,2012(10):1429-1430. |
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| Authors: | Chen Yinghong Dong Fang Jiang Xiangzhi Jiang Ruizhi and Wang Ying |
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| Institution: | 1(1.Jilin Academy of Traditional Chinese Medicine and Material Medical Sciences,Changchun 130012,China;2.Japan-China Union Hospital) |
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| Abstract: | Objective: To establish an HPLC method for the determination of rutin and hyperoside in Hypericum perforatum L..Method: A Diamonsil C18 column(250 mm×4.6 mm,5 μm) was eluted by the mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.4% acetic acid(1∶ 1∶ 18∶ 80) at the column temperature of 30℃.The flow rate was 0.7 ml·min-1 and detection wavelength was 358 nm.Result: Rutin was linear within the range of 0.206-1.236 μg with a regression coefficient of 0.999 9,and the average recovery was 98.79% with RSD of 0.91%.Hyperoside was linear within the range of 0.144-0.864 μg with a regression coefficient of 0.999 8,and the average recovery was 98.57% with RSD of 0.94%.Conclusion: The method is quick,simple and sensitive. |
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| Keywords: | Hypericum perforatum L Rutin Hyperoside HPLC |
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