| ~(125)碘-绒毛膜促性腺激素的电泳纯化及分析 |
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| 引用本文： | 李栗,雷幼导,张敏.~(125)碘-绒毛膜促性腺激素的电泳纯化及分析[J].四川大学学报(医学版),1993(3). |
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| 作者姓名： | 李栗 雷幼导 张敏 |
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| 作者单位： | 华西医科大学基础医学院同位素室
(李栗，雷幼导)，华西医科大学基础医学院同位素室(张敏) |
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| 摘 要： | 标记抗原在贮存过程中,由于受幅射分解和脱碘影响而使免疫活性和生物活性降低。我们对自制的~(125)碘-绒毛膜促性腺激素进行了实验观察。结果显示:贮存1月后,其免疫活性和生物活性分别降低了33.1％和44.0％;经电泳纯化后的标记物的免疫活性和生物活性明显提高,分别达到了最初的90.5％和95.3％。
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| 关 键 词： | ~(125)碘-绒毛膜促性腺激素 聚丙烯酰胺凝胶电泳 免疫活性 生物活性 |
Study on ~(125)I-Labeled Human Chorinic Gonadotropin after Purification Through Gel Electrophoresis |
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| Li Li,Lei Youdao,Zhang Min Central Laboratory of Isotopes.Study on ~(125)I-Labeled Human Chorinic Gonadotropin after Purification Through Gel Electrophoresis[J].Journal of West China University of Medical Sciences,1993(3). |
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| Authors: | Li Li Lei Youdao Zhang Min Central Laboratory of Isotopes |
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| Institution: | Li Li;Lei Youdao;Zhang Min Central Laboratory of Isotopes |
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| Abstract: | Radioiodination of HCGwith ~(125)I Na using chloramine-T is widelyemployed. However, ~(125)I-HCG damages instorage and thus results in decrease ofimmunological and biological activities.When ~(125)I-HCG was in store as long as 30days, the immunological activity and bio-logical activity decreased from 100% to33. 1% and 44. 0%, respectively. We usedpolyacrylamide gel electrophoresis tostore for 30 days since radiolabeling, andwe found the binding capacities of purified~(125)I-HCG increased. The combination ratesof purified ~(125)I-HCG with antibody and re-ceptors accounted for 90. 5% and 95. 3% ofthose of ~(125)I-HCG 1 day after radiolabel-ing. These results suggest that ~(125)I-HCGwhich has been stored for a period sinceradiolabeling should be purified beforeuse. |
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| Keywords: | ~(125)I-labeled HCG Polyacrylamide gel electrophoresis Immunological activity Biological activity |
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