精氨酸加压素对沙土鼠急性缺血性脑水肿的作用

本文探讨了精氨酸加压素(AVP)对急性缺血性脑水肿的作用及其作用机理。结果表明,侧脑室注射AVP后,缺血性皮层水肿显著加重,且这一作用不能被钙通道拮抗剂所阻断。侧脑室注射AVP抗血清、V_2及V_1/V_2型AVP受体拮抗剂均显著减轻缺血性皮层水肿。此外,侧脑室注射AVP后,缺血皮屋Na~+-K~+ATP酶活力显著降低;皮层等脑区cAMP含量显著升高,下丘脑和纹状体cGMP含量亦显著升高。提示AVP可能是通过cAMP、cGMP介导的AVP受体的作用,产生抑制脑细胞膜Na~+-K~+ATP酶活力的效应,从而促进缺血性脑水肿的形成。

The Effect of Arginine Vasopressin ( AVP ) on Acute Ischemic Brain Edema in Gerbil

The purpose of this experiments is to study the role of arginine vasopressin (AVP)iu acute ischemic brain edema and its mechanism. The results showde that intracerebroventricular injection ( ICV ) of AVP exacerbated the ischemic corticalc edema,and the calcium entry Mocker couldn' t block this role of AVP in ischemi brain edema. ICV of AVP antiserum,m V2 and V1/V2 receptor antagonist respectively decreased significantly the ischemic cortical edema. In addition, the Na + -K+ATPase activity of ischemic cortex was significantly decreased, and the contents of cAMP in cortex, hy pothalamus, staiatumand pons-medulla were markedly increased, and the contents of cGMP in hypothalamus and staiatum alsoincreased after ICV of AVP. These suggested that AVP was involved in the pa-thophysiologic process of acute ischemic brain edema, and its mechanism mightbe the effect of AVP on AVP receptor mediated by cAMP, cGMP, and that in turn inhibited the Na+-K+ ATPase activity of brain cell membrane,then exaggerated the formation of ischemic brain edema