X射线照射对原代培养海马神经元突起生长的作用

目的 探讨X射线照射对离体培养原代海马神经元突起生长的作用。方法 采用离体的方式培养原代海马神经元,分别给予0、2、4、8、10和12 Gy剂量照射,在照射后第1天和第3天,采用MTT法检测电离辐射对原代海马神经元死亡的影响,免疫荧光染色检测电离辐射对原代海马神经元神经突起的影响。结果 在照射后第1天和第3天,随着剂量的增加,海马神经元细胞死亡增多,差异有统计学意义(F=123.068、43.370,P<0.05),但4、8、10和12 Gy照射组间差异无统计学意义(P>0.05)。不同剂量照射后第1天,神经突起长度、总树突分支长度(TDBL)差异均有统计学意义(F=9.169、7.856,P<0.05),照后第3天神经突起长度、TDBL和总树突分支点数(TDBTN)差异均有统计学意义(F=23.797、6.565、6.021,P<0.05)。结论 X射线照射对离体培养的原代海马神经元突起的生长具有抑制作用。

Effect of X-ray irradiation on the neurites growth of primary hippocampal neurons

Objective To investigate the effect of X-ray irradiation on neurites growth of primary hippocampal neurons in vitro. Methods Primary hippocampal neuron culture in vitro were irradiated with 0, 2, 4, 8, 10, 12 Gy of X-rays. In the first day and the third day after irradiation, the cell death of primary hippocampal neurons was detected by MTT method, and the morphological changes of primary hippocampal neuronal neurites were detected with immunofluorescence staining method. Results In the first day and third day after irradiation, the cell death of hippocampal neurons increased significantly (F=123.068, 43.370,P<0.05), but there were no significant difference among 4, 8, 10, and 12 Gy irradiation groups. Immunofluorescence staining showed that, in the first day after irradiation, the neurite length and total dendritic branch length (TDBL) were significantly changed (F=9.169, 7.856, P<0.05), and in the third day after radiation,the neurite length, TDBL and total dendritic branch tip number (TDBTN) were also altered (F=23.797, 6.565, 6.021, P<0.05). Conclusion X-ray irradiation can inhibit the growth of neurites in the primary hippocampal neurons in vitro.