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| 我国4种白蛉的核糖体内转录间隔2区PCR-RFLP多态性分析 | |
| 引用本文： | Gu DA,Zhang Y,Lan QX. 我国4种白蛉的核糖体内转录间隔2区PCR-RFLP多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2012, 30(2): 100-1, 108 |
| 作者姓名： | Gu DA Zhang Y Lan QX |
| 作者单位： | 中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心;苏州市疾病预防控制中心 |
| 基金项目： | 国家科技重大专项(No.2008ZX10004-010)~~ |
| 摘 要： | 采用PCR-RFLP方法对我国内脏利什曼病传播媒介中华白蛉(Phlebotomus chinensis)、吴氏白蛉(Ph.wui)、长管白蛉(Ph.longiductus)和亚历山大白蛉(Ph.alexandri)等4种白蛉的核糖体内转录间隔2区(ITS2)进行分型。PCR-RFLP结果显示,以限制性内切酶DraⅠ酶切ITS2片段,可分出4种RFLP带型,即A为450 bp,B为154 bp/127 bp/93 bp/66 bp/10 bp,C为311 bp/80 bp/60 bp,D为353 bp/300 bp/160 bp/58 bp。结果显示,4种白蛉均只有1种RFLP带型,中华白蛉为AA型,亚历山大白蛉为BB型,吴氏白蛉为CC型,长管白蛉为DD型。利用PCR-RFLP方法可区分我国4种内脏利什曼病传播媒介的种类。 |
| 关 键 词： | 白蛉 内转录间隔2区(ITS2) 限制性片段长度多态性(RFLP) |
Analysis of polymorphism on ribosomal internal transcribed spacer 2 in four species of sandflies with PCR-RFLP |
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| Gu Deng-An,Zhang Yi,Lan Qin-Xian. Analysis of polymorphism on ribosomal internal transcribed spacer 2 in four species of sandflies with PCR-RFLP[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2012, 30(2): 100-1, 108 | |
| Authors: | Gu Deng-An Zhang Yi Lan Qin-Xian |
| Affiliation: | National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China. |
| Abstract: | Internal transcribed spacer 2 region in 4 speices of sanflies from China (Phlebotomus chinensis, Ph. wui, Ph. longiductus and Ph. alexandri) was genotyed by PCR-RFLP. Four kinds of RFLP band patterns were created by Dra I, that were A 450 bp, B 154 bp/127 bp/93 bp/66 bp/10 hp, C 311 bp/80 bp/60 bp, and D 353 bp/300 bp/160 bp/58 bp. There were identical pattern types in various sandflies, AA in Ph. chinensis, BB in Ph. alexandri, CC in Ph. wui, and DD in Ph. longiductus. The results indicated that PCR-RFLP can be used to identify the four speicies of Phlebotomus in China. |
| Keywords: | Sandfly Internal transcribed spacer 2(ITS2) Restriction fragment length polymorphism(RFLP) |
| 本文献已被 CNKI PubMed 等数据库收录！ | |