| 酶免疫法检测抗丙型肝炎病毒E2抗体 |
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| 引用本文： | 朱朝敏,吴朝栋,陶其敏. 酶免疫法检测抗丙型肝炎病毒E2抗体[J]. 中华检验医学杂志, 1999, 22(4) |
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| 作者姓名： | 朱朝敏 吴朝栋 陶其敏 |
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| 作者单位： | 北京医科大学人民医院肝病研究所 |
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| 基金项目： | 美国中华医学基金，国家九五攻关项目资助 |
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| 摘 要： | 目的 试用一种更敏感、更特异酶免疫法检测的丙型肝炎病毒（ Ｈ Ｃ Ｖ） 抗体。方法 将体外真核系统表达并纯化的 Ｈ Ｃ Ｖ Ｅ２ 糖蛋白作为包被抗原，用于２５７ 份丙型肝炎患者血清标本的抗 Ｅ２ 抗体检测。结果 成功利用 Ｈ Ｃ Ｖ 的 Ｅ２ 糖蛋白建立酶免疫法检测抗 Ｅ２ 抗体，在 Ｈ Ｃ Ｖ Ｒ Ｎ Ａ 阳性标本中，抗 Ｅ２ 抗体检出率为８２４２ ％ ，高于抗 Ｈ Ｃ Ｖ 抗体的检出率，两者比较差异有显著意义（ Ｐ＜ ００５） 。结论 证实抗 Ｅ２ 抗体的检测有较好的敏感性及特异性， Ｅ２ 糖蛋白可用于检测抗 Ｈ Ｃ Ｖ 抗体的新一代检测试剂。
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| 关 键 词： | 肝炎病毒组.丙型 糖蛋白类 免疫酶技术 |
Enzyme immune assay for detecting antibody against hepatitis C virus E2 glycoprotein |
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| Abstract: | Objective To detect antibody against E2 with E2 glycoprotein of hepatitis C virus as antigen for establishment and application of enzyme immune assay.Methods E2 glycoprotein expressed in mammalian cell was purified and used as antigen for detection of antibody against E2 in 257 cases of clinical samples.Results It was first time to use EIA method for detection of anti E2 antibody by purified E2 glycoprotein in China. Positive rate of anti E2 in HCV RNA positive samples was 82.42%, which was higher than that of anti HCV. The difference was significant ( P <0.05).Conclusion The detection system showed good sensitivity and specificity. E2 glycoprotein may be useful for the development of new anti HCV reagents. |
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| Keywords: | Hepatitics C viruses Glycoproteins Immunoenzyme techniques |
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