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| PCR-CTPP技术在MDR1基因SNP分型中的应用 | |
| 引用本文： | 凌烈锋,董斌,聂刘旺.PCR-CTPP技术在MDR1基因SNP分型中的应用[J].皖南医学院学报,2010,29(6):405-408. |
| 作者姓名： | 凌烈锋 董斌 聂刘旺 |
| 作者单位： | 1. 安徽师范大学,生命科学院,安徽,芜湖,241001;皖南医学院,生化教研室,安徽,芜湖,241002 2. 合肥市第一人民医院,神经内科,安徽,合肥,230022 3. 安徽师范大学,生命科学院,安徽,芜湖,241001 |
| 基金项目： | 安徽省高校省级自然科学研究项目，皖南医学院中青年科研基金项目 |
| 摘 要： | 目的:建立两对引物-聚合酶链反应(PCR-CTPP)技术改进体系-两步法PCR-CTPP,研究其在MDR1基因SNP的快速分型中的应用价值。方法:采用两步法PCR-CTPP技术对158例的癫痫患者MDR1基因rs3789243和rs2235046进行检测分型,并以DNA测序验证基因分型结果。结果:通过两步法PCR-CTPP得到的基因分型结果与DNA测序得到的基因分型结果完全一致。结论:改进的两步法PCR-CTPP技术是一种可靠、快速的对基因SNP分型检测方法,能在普通实验室广泛应用,有助于基因SNP的分型研究。 |
| 关 键 词： | PCR-CTPP MDR1基因 SNP |
Applying polymerase chain reaction with confronting two-pair primers to genotyping the MDR1 gene single nucleotide polymorphism |
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| LING Lie-feng,DONG Bin,NIE Liu-wang.Applying polymerase chain reaction with confronting two-pair primers to genotyping the MDR1 gene single nucleotide polymorphism[J].Acta Academiae Medicinae Wannan,2010,29(6):405-408. | |
| Authors: | LING Lie-feng DONG Bin NIE Liu-wang |
| Institution: | School of Life Science,Anhui Normal University,Wuhu 241001,China;Department of Biochemistry,Wannan Medical University,Wuhu 241002,China |
| Abstract: | Objective：To evaluate the value of improved two-step polymerase chain reaction with confronting two-pair primers（PCR-CTPP）for quick genotyping MDR1 gene single nucleotide polymorphism（SNP）.Methods：MDR1 gene polymorphism rs3789243 and rs2235046 were detected by two-step PCR-CTPP in 158 epileptics and genotyped with DNA sequence analysis.Results：The results of genotyping by two-step PCR-CTPP were consistent with those conducted by DNA sequence analysis.Conclusion：The improved two-step PCR-CTPP appears a fast and reliable method for gene genotyping and may be recommended in wider general laboratory use in research of gene SNP genotyping. |
| Keywords: | PCR-CTPP SNP |
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