A method for the quantitative estimation of drugs on the isolated intact trachea

A method is described for the detection and comparison of substances active on tracheal smooth muscle. Several pharmacological agents affecting smooth muscle have been tested using the technique. Sensitivity was high, results reproducible and linear log dose-response relationships obtained over a wide dosage range. The method has been used to estimate the concentrations of adrenaline, acetylcholine and histamine in test solutions of these compounds.