RP-HPLC法测定氨茶碱血清浓度

目的:建立测定氨茶碱血清浓度的方法。方法:采用反相高效液相色谱法,色谱柱为BDS C18,流动相为甲醇-水(15∶85),柱温为35 ℃,流速为1 mL·min-1,检测波长为275 nm。结果:氨茶碱有效成分茶碱血药浓度在0.5～42.5 μg·mL-1范围内线性关系良好(r=0.999 9),最低检测限为0.5 μg·mL-1;方法回收率为91.60%～95.00%,提取回收率为68.04%～70.77%;日内RSD≤2.20%,日间RSD≤3.28%。结论:本方法简便、准确、灵敏、重复性好,可用于氨茶碱血药浓度监测。

Determination of Aminophylline Concentration in Serum by RP-HPLC

OBJECTIVE：To develop the method for the determination of aminophylline concentration in serum. METHODS： RP-HPLC method was adopted. The determination was performed on BDS C18 column with mobile phase consisted of methanol-water （15∶85） at a flow rate of 1 mL·min-1. The detection wavelength was set at 275 nm and the column temperature was 35 ℃. RESULTS： The linear range of theophylline was 0.5～42.5 μg·mL-1（r=0.999 9） with lowest detection limit of 0.5 μg·mL-1. The extraction recoveries were 68.04%～70.77% and methodology recoveries were 91.60%～95.00%. The inter-day RSD was less than 2.20%, and intra-day RSD was less than 3.28%. CONCLUSION： The method developed is simple, accurate, sensitive and reproducible, and it is suitable for the blood monitoring of aminophylline.