半乳糖凝素-3和间皮瘤相关抗体-1在甲状腺结节中的表达及其临床意义

目的 观察半乳糖凝素-3(Galectin-3)和间皮瘤相关抗体-1(HBME-1)在甲状腺良恶性结节中的表达差异,探讨二者的临床应用价值.方法 采用免疫组化SP法,检测30例甲状腺乳头状癌(PTC)和 30例结节性甲状腺肿(NG)石蜡标本中Galectin-3和HBME-1的表达情况.结果 Galectin-3,HBME-1及二者联合标记在PTC组的检出率分别为93.3%(28/30),83.3%(25/30),100.0%(30/30),均高于NG组[20.0%(6/30),6.7%(2/30),26.7%(8/30)],组问比较差异有统计学意义(X2值分别为32.85,35.65,34.74,P＜0.01).Galectin-3,HBME-1及联合标记的灵敏度分别为93.3%,83.3%,100.0%,特异度分别为80.0%,93.3%,73.3%,阳性预测值分别为82.3%,92.6%,78.9%,阴性预测值分别为92.3%,84.8%,100.0%,诊断准确率分别为86.7%,88.3%,86.7%.结论 Galeetin-3和HBME-1的检测可用于PFC和NG的鉴别,而联合检测可提高PTC的检出率.

Significance of Galectin-3 and HBME-1 expression in differential diagnosis of thyroid nodules

Objective To study the difference of galectin-3 and HBME-1 expression between benign and malignant thvreid nodules and to evaluate their clinical value.Methods Using immunohistochemical SP method,the paraffin-embedded tissues of 30 ewes papillary thyroid carcinoma(PTC)and 30 cases nodular goiter(NG) were examined to deteet the expression of Galectin-3 and HBME-1.Results The rates of positive staining of Galectin-3.HBME-1.Galectin-3 combined with HBME-1 in PTC were 93.3%(28/30),83.3%(25/30)and 100.0%(30/30),respectivdy,were higher than that of NG[20.0%(6/30),6.7%(2/30)and 26.7%(8/30)].There were significant differences between PTC and NG(X2＝32.85,35.65,34.74,all P＜0.01).The sensitivity,specificity,positive predietive value.negative predictive value and accuracy rate of using Galectin-3,HBME-1,Galectin-3 combined with HBME-1 in differentiation NG from FrIE were 93.3%,83.3%,100.0%;80.0%,93.3%,73.3%;82.3%,92.6%,78.9%:92.3%,84.8%,1 00%;86.7%,88.3%,86.7%,respectively.Conclusion Immunohistochemical stains of Galectin-3 and HBME-1 may be used as two lnarkel's for differentiating PTC and NG,it is especially sensitive when the two markers are jointly used.