| 吴茱萸碱在诱导小鼠纤维肉瘤L929细胞凋亡过程中对细胞周期的阻滞作用 |
| |
| 引用本文： | 张莹,张起辉,吴立军,田代真一,小野寺敏,池岛乔. 吴茱萸碱在诱导小鼠纤维肉瘤L929细胞凋亡过程中对细胞周期的阻滞作用[J]. 中国中西医结合杂志, 2004, 0(Z1) |
| |
| 作者姓名： | 张莹 张起辉 吴立军 田代真一 小野寺敏 池岛乔 |
| |
| 作者单位： | 沈阳药科大学中日医药研究所，沈阳药科大学天然药物研究室，沈阳药科大学天然药物研究室，日本昭和药科大学病态科学教研室，日本昭和药科大学病态科学教研室，沈阳药科大学中日医药研究所 沈阳110016)，沈阳110016) |
| |
| 摘 要： | 目的研究吴茱萸碱体外诱导 L929细胞死亡的机制。方法用 MTT 法测定吴茱萸碱对 L929的细胞毒作用。通过倒置显微镜,荧光染色,观察 L929在吴茱萸碱作用下的形态学变化,用 LDH 活力测定法证明凋亡途径,用流式细胞分析技术研究昊茱萸碱对细胞周期的影响。结果 MTT 法测定不同浓度吴茱萸碱明显抑制 L929生长,并具有剂量依赖性。吴茱萸碱可诱导 L929凋亡,呈现凋亡小体,亚二倍体峰出现,但无典型因凋亡引起的 DNA 梯状泳带。低剂量5μmol·L~(-1)吴茱萸碱可将细胞周期阻滞在 G_2/M 期,而高剂量20μmol·L~(-1)吴茱萸碱可将细胞周期阻滞在 G_0/G_1 期。结论在吴茱萸碱诱导 L929凋亡过程中低剂量将细胞周期阻滞在 G_2/M 期,而高剂量则引起 G_0/G_1期阻滞。
|
| 关 键 词： | 吴茱萸碱 小鼠纤维肉瘤 L929细胞 细胞凋亡 细胞周期 |
Cell Cycle Arrest in Mouse Fibrosarcoma L929 Cell Apoptosis Induced by Evodiamine |
| |
| ZHANG Ying ZHANG Qihui WU Li-jun China-Japan Research Institute of Medical and Pharmaceutical Sciences,Shenyang Pharmaceutical University,Shenyang. Cell Cycle Arrest in Mouse Fibrosarcoma L929 Cell Apoptosis Induced by Evodiamine[J]. Chinese journal of integrated traditional and Western medicine, 2004, 0(Z1) |
| |
| Authors: | ZHANG Ying ZHANG Qihui WU Li-jun China-Japan Research Institute of Medical Pharmaceutical Sciences Shenyang Pharmaceutical University Shenyang |
| |
| Abstract: | Objective To study the mechanism of L929 cell death induced by Evodiamine in vitro.Methods Cytotoxicity of L929 induced by Evodiamine was determined by MTT method.Morphological changes of L929 cells under Evodiamine in- duction by inverted microscope and fluorescent staining.Apoptosis pathway was proved by LDH activity determination.Ef- fect of Evodiamine on cell cycle was studied by flow cytometry analysis technique.Results MTY method showed that Evodiamine of different concentrations could obviously inhib... |
| |
| Keywords: | Evodiamine mouse fibrosarcoma L1929 cell apoptosis cell cycle |
| 本文献已被 CNKI 维普 等数据库收录！ |
