| 标本处理方法对多聚酶链反应的影响 |
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| 引用本文： | 唐振亚,赵西平,喻植群,汪由坤,李方和,杨东亮,郝连杰.标本处理方法对多聚酶链反应的影响[J].华中科技大学学报(医学版),1995(3). |
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| 作者姓名： | 唐振亚 赵西平 喻植群 汪由坤 李方和 杨东亮 郝连杰 |
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| 作者单位： | 同济医科大学附属同济医院临床免疫学研究室 |
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| 摘 要： | 对多聚酶链反应（ＰＣＲ）扩增条件相同情况下，５种标本处理方法ＨＢＶＤＮＡ检测结果进行了比较。结果证实，酶消化法、碱变性法和微波法ＨＢＶＤＮＡ检出率（均为７１．８８％，４６／６４）高于直接法和热变性法（均为６２．５％，４０／６４），而且酶消化法、碱变性法和微波法的敏感性明显高于直接法和热变性法。综合比较这５种ＰＣＲ方法，碱变性法和微波法不仅敏感性高、简便和快速，而且抗污染性强、结果稳定可靠，值得推广应用。
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| 关 键 词： | 肝炎病毒 多聚酶链反应 方法学 |
Influence of Sample Treatment Methods on the Polymerase Chain Reaction |
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| Tang Zhenya,Zhao Xiping,Yu Zhiqun,et al.Influence of Sample Treatment Methods on the Polymerase Chain Reaction[J].Journal of Huazhong University of Science and Technology(Health Sciences),1995(3). |
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| Authors: | Tang Zhenya Zhao Xiping Yu Zhiqun |
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| Abstract: | Under the same amplification condition of polymerase chain reaction (PCR),five different sample treatment methods for detection of HBV DNA were compared.The results showed that 71.88%(46/64) of samples was detected HBV DNA positive by enzyme digestion,NaOH denaturation and microwave methods,while only 62.5%(40/64) was detected HBV DNA positive by direct detection and heat denaturation methods,We demonstrated that enzyme digestion,NaOH denaturation and microwave methods were more sensitive than direct detection and heat denaturation methods.Compared with other methods,NaOH denaturation and microwave methods were not only sensitive,simple and quick,but also contamination-free and reliable. |
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| Keywords: | hepatitis virus polymerase chain reaction methodology |
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