肝硬化门静脉高压模型犬舌下络脉形态和舌下组织血管内皮生长因子及低氧诱导因子1α的表达

目的：观察肝硬化门静脉高压犬舌下络脉变化及舌下组织血管内皮生长因子（vascular endothelial growth factor,VEGF）和低氧诱导因子1α（hypoxia-inducible factor1α,HIF-1α）的表达情况。方法：将12只Beagle犬随机分为正常组和模型组。用二甲基亚硝胺（dimethylnitrosamine,DMN）诱导肝硬化门静脉高压,观察Beagle犬舌下络脉形态、颜色;生物电记录系统测定其门静脉压力;免疫组织化学EnVision法检测犬舌下组织VEGF及HIF-1α蛋白的表达。结果：模型组Beagle犬舌下血管形态学和颜色较正常组有明显改变。免疫组织化学结果显示,正常对照组犬舌下血管VEGF、HIF-1α几乎无表达;模型组犬舌下组织VEGF、HIF-1α蛋白表达显著增高,差异有统计学意义（P〈0.01）。结论：门静脉压力变化可能是肝硬化门静脉高压模型犬异常舌下络脉形成的一个重要因素,舌下血管VEGF和HIF-1α蛋白表达增加可能参与了这一过程。

Characteristics of sublingual vein and expressions of vascular endothelial growth factor and hypoxia-inducible factor la proteins in sublingual tissues of Beagle dogs with portal hypertension

Objective： To observe sublingual vein characteristics and the expressions of vascular endothelial growth factor （VEGF） and hypoxia-inducible factor 1α （HIF-1α） proteins in sublingual tissues of Beagle dogs with cirrhotic portal hypertension. Methods： Twelve Beagle dogs were randomly divided into normal control group and cirrhotic portal hypertension group. There were 6 dogs in each group. A canine model of cirrhosis portal hypertension was established by injecting dimethylnitrosamine （DMN） into portal vein once a week for 7 weeks. The characteristics of sublingual vein were observed. Portal venous pressure was measured by using bioelectric recording techniques. The expressions of VEGF and HIF-1α proteins in sublingual vein were detected by immunohistochemical method.
Results： The shape and color of sublingual vein in beagle dogs in the cirrhotic portal hypertension group changed obviously as compared with the normal control group. Immunohistochemical results showed that there were almost no expressions of VEGF and HIF-1α group; however, the expressions of VEGF and HIF-1α proteins in sublingual tissues in the normal contro proteins in sublingual tissues in the cirrhotic porta hypertension group significantly increased.
Conclusion： Changes of portal pressure may lead to the formation of the abnormal sublingual vein by increasing the expressions of VFGF and HIF-1α proteins in sublingual tissues of Beagle dogs with portal hypertension.