HPLC法同时测定四逆汤中3 种有效成分的含量

 目的建立同时测定四逆汤中乌头碱、新乌头碱和次乌头碱含量的HPLC 法。方法采用HPLC 色谱法，Eclipse XDB-
C18 色谱柱（4.6 mm*150 mm，5 μm），流动相甲醇-0.1%三乙胺（65:35），流速为1.0 mL·min-1，柱温为30益，检测波长为240 nm。
结果乌头碱、新乌头碱、次乌头碱分别在0.120 4～6.02 μg（ =0.9997）、0.041 2～2.06 μg（ =0.999 5）、0.080 8～4.04 μg（ =
0.999 9）范围内与峰面积呈良好的线性关系；平均回收率分别为100.74%（RSD=1.88%）、99.9%（RSD=2.98%）、102.9%（RSD=
1.32%）。结论该方法简便可行、重现性好、可作为四逆汤的质量控制方法。

Simultaneous Quantification of three active compounds in Sini Tang by HPLC

Objective To establish an HPLC（High Performance Liquid Chromatography）method for simultaneous quantification of aconi-
tine，mesaconitine and hypaconitine in Sini tang. Methods HPLC was performed on a C18 column （4.6 mm～150 mm，5 μm）with
methanol-0.1% triethylamine （65～35）as mobile phase. The flow rate was 1.0 mL·min-1，column temperature was fixed at 30 ，and the
wave length of detection was set at 240 nm. Results For aconitine，mesaconitine and hypaconitine，the linear ranges were 0.120 4～6.02
μg （ =0.999 7），0.0412～2.06 μg （ =0.999 5）and 0.080 8～4.04 μg （ =0.999 9），and their average recovery rates were 100.74%
（RSD=1.88%），99.9%（RSD=2.98%）and 102.9%（RSD=1.32%），respectively. Conclusion The method could be used for the quality
control of Sini Tang，which was available with a good reproducibility.