| HPLC法测定四方胃胶囊中盐酸小檗碱的含量 |
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| 引用本文： | 郭冬萍,梁其彬. HPLC法测定四方胃胶囊中盐酸小檗碱的含量[J]. 中国中医药信息杂志, 2008, 15(6): 50-51 |
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| 作者姓名： | 郭冬萍 梁其彬 |
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| 作者单位： | 1. 广东省中医院药学部,广东,广州510120
2. 广州中医药大学第一临床医学院,广东广州,510120 |
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| 摘 要： | 目的建立四方胃胶囊中盐酸小檗碱的含量测定方法。方法采用HPLC法，以十八烷基硅烷键合硅胶为填充剂，以乙腈-0．05mol／L磷酸二氢钾溶液（25：75）为流动相，检测波长230nm。理论塔板数按盐酸小檗碱计算应不低于3000。结果该含量测定方法线性良好，线性范围0．2016～1．008μg（，=0．9998）；加样回收率为98．7％，RSD=1．24％（n=5）。结论该方法简单、准确，分离度及重复性好，可用于四方胃胶囊的质量控制。
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| 关 键 词： | 高效液相色谱法 四方胃胶囊 盐酸小檗碱 含量测定 |
Content Determinate of Berberine Hydrochloride in Sifangwei Capsules by HPLC |
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| GUO Dong-ping,LIANG Qi-bin. Content Determinate of Berberine Hydrochloride in Sifangwei Capsules by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2008, 15(6): 50-51 |
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| Authors: | GUO Dong-ping LIANG Qi-bin |
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| Affiliation: | GUO Dong-ping , LIANG Qi-bin (1.Guangdong Hospital of TCM, Guangzhou 510120, China; 2.The First Clinical Medical College, Guangzhou University of TCM, Guangzhou 510120, China) |
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| Abstract: | Objective To establish the content determination method of berberine hydrochloride in Sffangwei capsules. Methods HPLC method was used with octadecylsilane chemically bonded silica as the filler, acetonitrile-0.05 mol/L KH2PO4 solution （25 ; 75） as the mobile phase, detective wavelength was 230 nm. The number of theoretical plates should not be less than 3 000. Results The content determination method has a good linear relation at the range of 0.201 641.008 pg （r=0.999 8）. The recovery rate was 98.7%, RSD=1.24% （n =5）. Conclusion The method is simple, accurate, with good separation and reproducibility, and can be used for the quality control of Sffangwei capsules. |
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| Keywords: | HPLC Sffangweicapsules berberine hydrochloride content determination |
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