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| 虫草属EST-SSR标记系统的建立研究 | |
| 引用本文： | 管俊娇,虞泓,解云峰,左世梅,马荣锋,曾文波. 虫草属EST-SSR标记系统的建立研究[J]. 中国中药杂志, 2011, 36(13): 1711-1717 |
| 作者姓名： | 管俊娇 虞泓 解云峰 左世梅 马荣锋 曾文波 |
| 作者单位： | 1. 云南大学中草药生物资源研究所云百草实验室,云南昆明,650091;云南省农业科学院质量标准与检测技术研究所,云南昆明,650205 2. 云南大学中草药生物资源研究所云百草实验室,云南昆明,650091 |
| 基金项目： | 云南省自然科学基金重点项目(2008CC019) |
| 摘 要： | 目的:通过球孢虫草、蛹虫草EST设计EST-SSR引物,建立虫草属EST-SSR标记系统.方法:从NCBI公共数据库下载获得虫草EST,利用Sequece Seiners 1.2软件去除冗余序列并设计引物,进行PAGE电泳.结果:通过去除EST总序列中低质量的和冗余的序列后,得到全长为2 953 173 bp的4 556条无冗余球孢虫草EST.从中发掘出718个EST-SSR,分布于616条EST中,出现频率是15.8％.平均分布频率是每4 096 bp出现1个,三核苷酸重复序列有419个,是出现最多的重复类型.蛹虫草EST去冗余后得到1 363条无冗余EST,共含有1 117个EST-SSR,出现频率为81.95％,出现最多的重复类型是A核苷酸重复.根据球孢虫草EST-SSR序列,设计合成50对引物,有扩增产物的引物为34对,占总设计引物数的68％.根据蛹虫草EST-SSR,设计合成40对引物,有扩增产物的引物为39对,占总设计引物数的97.5％.基于SSR标记进行聚类分析,7种虫草无性型均能分开,且分为4支.结论:虫草属EST-SSR出现频率较高、类型较丰富、多态性潜能较高,具有较高的利用价值.球孢虫草和蛹虫草EST开发的SSR标记在虫草属有较好的转移性与通用性,可以很好的应用于虫草种间遗传关系的研究.应用虫草物种EST建立分子标记是一条简便而又有效的途径. |
| 关 键 词： | 虫草属 球孢虫草 蛹虫草 EST-SSR 引物设计 |
| 收稿时间： | 2010-12-09 |
Study of EST-SSR marker system of Cordyceps |
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| GUAN Junjiao,YU Hong,XIE Yunfeng,ZUO Shimei,MA Rongfeng and ZENG Wenbo. Study of EST-SSR marker system of Cordyceps[J]. China Journal of Chinese Materia Medica, 2011, 36(13): 1711-1717 | |
| Authors: | GUAN Junjiao YU Hong XIE Yunfeng ZUO Shimei MA Rongfeng ZENG Wenbo |
| Affiliation: | Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;Institute of Agricultural Quality Standard and Detecting Technology of Yunnan Academy of Agricultural Sciences, Kunming 650205, China;Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China |
| Abstract: | Objective: To establish the EST-SSR marker system for Cordyceps by using ESTs of C.bassiana and C.militaris.Method: The ESTs of Cordyceps were downloaded from the public database of NCBI,and the redundant ESTs with low quality were removed.The EST-SSR primers were designed by Sequece Seiner 1.2.And the primers were screened through PAGE-Electrophoresis.Result: The 4 556 non-redundant ESTs which from C.bassiana with total length of 2 953 173 bp were selected.718 EST-SSRs distributed in 616 ESTs were totally ... |
| Keywords: | Cordyceps Cordyceps bassiana Cordyceps militaris EST-SSR primer design |
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