| RP-HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸 |
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| 引用本文： | 许栋明,程可建.RP-HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸[J].中国中药杂志,2011,36(1):45-47. |
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| 作者姓名： | 许栋明 程可建 |
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| 作者单位： | 1. 科技部科技型中小企业技术创新基金管理中心,北京,100038
2. 北大世佳研究中心,北京,100084 |
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| 摘 要： | 目的:建立HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸含量的方法.方法:DIKMA Diamonsil(2)-C18柱(4.6 mm×250 mm,5 μm);流动相乙腈(A)-0.1%磷酸溶液(B),线性梯度洗脱;检测波长237,283 nm;柱温25℃;流速1.0 mL·min-1;进样量10μL.结果:甘草苷、柚皮苷、橙皮苷和甘草酸铵的进样量与峰面积,分别在0.019 9～0.119(r=0.999 7),0.180～1.08(r=0.999 7),0.146～0.873(r=0.999 8),0.0393～0.236μg(r=0.999 7)呈良好的线性关系;平均加样同收率依次为97.7%,97.7%,97.1%,98.5%,RSD 1.4%,2.0%,2.0%,1.9%.结论:该方法快速,简便,重复性好,适合于同时测定温胆汤样品中甘草苷、柚皮苷、橙皮苷和甘草酸的含量.
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| 关 键 词： | 温胆汤 甘草苷 柚皮苷 橙皮苷 甘草酸 高效液相色谱法 |
| 收稿时间： | 2010/7/13 0:00:00 |
Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC |
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| XU Dongming and CHENG Kejian.Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC[J].China Journal of Chinese Materia Medica,2011,36(1):45-47. |
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| Authors: | XU Dongming and CHENG Kejian |
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| Institution: | Science and Technology Innovation of Small and Mid-sized Enterprise Fund Management Center, Ministry of Science and Technology, Beijing 10038, China;Bescholor Research Center, Peking University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | extraction of Wendan formula liquiritin naringin hesperidin glycyrrhizic acid HPLC |
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