| 三角酵母D-氨基酸氧化酶的固定化研究 |
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| 引用本文： | 陈少欣,吴文琼,刘晨,史炳照. 三角酵母D-氨基酸氧化酶的固定化研究[J]. 中国医药工业杂志, 2003, 34(2): 69-72 |
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| 作者姓名： | 陈少欣 吴文琼 刘晨 史炳照 |
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| 作者单位： | 上海医药工业研究院,上海,200040 |
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| 基金项目： | 国家经贸委资助项目 (无编号 ) |
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| 摘 要： | 通过共价结合的方法把三角酵母D—氨基酸氧化酶(DAO)分别固定在三种大孔高聚物和二种多糖的载体上，结果表明多糖类载体壳聚糖和Sepharose 4B比高聚物更能有效地固定化DA0。经过固定化后，壳聚糖和环氧载体Epecust固定化酶对温度和pH的稳定性提高，表现米氏常数增大。在分批式头孢菌素C氧化脱氨的反应中，固定化酶表现出良好的操作稳定性，戊二酸单酰基—7—氨基头孢霉烷酸的得率为93％。
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| 关 键 词： | 三角酵母 D－氨基酸氧化酶 固定化 DAO 大孔高聚物 多糖类 载体 壳聚糖 |
| 文章编号： | 1001-8255(2003)02-0069-04 |
| 修稿时间： | 2002-09-01 |
Immobilization of D-amino Acid Oxidase from T. variabilis |
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| CHEN Shao-Xin,WU Wen-Qiong,LIU Chen,SHI Bing-Zhao. Immobilization of D-amino Acid Oxidase from T. variabilis[J]. , 2003, 34(2): 69-72 |
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| Authors: | CHEN Shao-Xin WU Wen-Qiong LIU Chen SHI Bing-Zhao |
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| Abstract: | D-amino acid oxidase (DAO) from Trigonopsis variabilis was convalently linked to three macroporous polymers and two polysaccharide supports, respectively. The coupling to the latter, such as chitosan and Sepharose 4B, was more effective than that to the former. The immobilized DAO on chitosan and Epecust exhibited an enhancement of stability against thermal and pH inactivation as well as an increase in the apparent Michaelis constant (K m). The immobilized enzymes used in repeated reaction cycles for deamination of cephalosporin C showed an appreciable operational stability. Yield of glutaryl-7-ACA was about 93% in batch-type production. |
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| Keywords: | D-amino acid oxidase immobilized enzyme Cephalosporin C Glutaryl-7-ACA |
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