高机械指数超声辐照微泡对结肠癌细胞增殖及凋亡的影响

目的 探讨高机械指数超声造影对结肠癌细胞增殖、凋亡及细胞周期的影响.方法 体外培养结肠癌细胞株(Lovo细胞).分为对照组、微泡+超声组、单纯超声辐照组和单纯微泡组.使用超声造影剂SonoVue,探头频率1.5 MHz,机械指数1.7进行超声间歇辐照,采用MTT法、流式细胞仪检测细胞周期及凋亡指数.结果 对照组、单纯辐照组及单纯微泡组的增殖能力、细胞周期与凋亡指数无明显差异(P>0.05);微泡+超声组与对照组比较,其增殖能力明显低于对照组(P<0.01),而凋亡指数明显高于对照组(P<0.01).结论 高机械指数超声辐照微泡击破效应对结肠癌细胞的增殖有明显抑制作用,而对结肠癌细胞的凋亡有明显促进作用.

Effect of high mechanical index contrast-enhanced ultrasound on the proliferation and apoptosis of colonic cancer cells

Objective To investigate the effects of high mechanical index contrast-enhanced ultrasound on the proliferation and apoptosis of colonic cancer cells.Methods Lovo eell were cultured in vitro,and this study was divided into control group,simple contrast agent group,microbubble plus ultrasound radiation group and simple ultrasound radiation group.Contrast agent SonoVue and uhransound(frequency 1.5 MHz and MI 1.7)were used.Proliferation of Lovo cells were detected by MTT assay,and apoptotic index by flowcytometry(FCM),respectively.Results There weren't obvious differences in proliferation,apoptosis and cells cycle between the control group,the simple ultrasound radiation group and the simple contrast agent group(P>0.05).There were obvious differences between the control group and the microbubble plus ultrasound radiation group in proliferation ability and apoptotic index(P<0.01).Conclusions High mechanical index ultrasound radiation can enhance the contrast agent SonoVue's destruction,inhibit the proliferation and promote apoptosis of tumor cells.