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| 当归枸杞制剂诱导脐血单个核细胞分化为肝细胞后人白蛋白的表达 | |
| 引用本文： | 傅京力,曾妍,龙则灵,唐晓鹏.当归枸杞制剂诱导脐血单个核细胞分化为肝细胞后人白蛋白的表达[J].中国组织工程研究与临床康复,2011,15(45):8418-8423. |
| 作者姓名： | 傅京力 曾妍 龙则灵 唐晓鹏 |
| 作者单位： | 1. 中南大学湘雅二医院肝病中心,中南大学肝病研究所,湖南省长沙市,410011;株洲市一医院感染内科,湖南省株洲市,412000 2. 湖南省劳动卫生职业病防治研究所附属医院,湖南省长沙市,410007 3. 中南大学湘雅二医院肝病中心,中南大学肝病研究所,湖南省长沙市,410011 |
| 基金项目： | 国家自然科学基金项目(39870651); 湖南省自然科学基金项目(04JJ6048)~~ |
| 摘 要： | 背景:部分中药能够促进造血干细胞的生长及分化。目的:观察当归、枸杞制剂促进人脐血单个核细胞生长分化为肝细胞的作用、作用条件及对肝衰竭的治疗效果。方法:分离人脐血单个核细胞,加入不同剂量的当归及枸杞制剂,比较不同剂量当归及枸杞制剂对脐血单个核细胞及其转化为肝细胞后人白蛋白mRNA及人甲胎蛋白mRNA表达的影响。腹腔内注射D-氨基半乳糖建立大鼠肝衰竭模型,随机分为生理盐水对照组、脐血单个核细胞组、脐血单个核细胞与当归、枸杞制剂灌胃和腹腔内注射免疫抑制剂不同组合8组,检测大鼠肝功能变化。结果与结论:90mg/L当归、40mg/L枸杞制剂可促进单个核细胞的增殖和分化,并加快脐血单个核细胞分化为肝细胞,两者联合无药物协同作用。单独应用当归与枸杞对大鼠肝损伤均有一定的修复作用,与单个核细胞联合均可促进单个核细胞向肝细胞分化,增强修复肝损伤的作用,并且当归的修复效果优于枸杞。加用环磷酰胺后并不能增强当归或枸杞联合单个核细胞的治疗效果。 |
| 关 键 词： | 当归 枸杞 脐血 单个核细胞 细胞分化 肝细胞 |
Angelica sinensis (oliv.) diels and lycium chinense mill preparations induce the differentiation of cord blood mononuclear cells into hepatocyts: Expression of human albumen |
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| Fu Jing-li,Zeng Yan,Long Ze-ling,Tang Xiao-peng.Angelica sinensis (oliv.) diels and lycium chinense mill preparations induce the differentiation of cord blood mononuclear cells into hepatocyts: Expression of human albumen[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(45):8418-8423. | |
| Authors: | Fu Jing-li Zeng Yan Long Ze-ling Tang Xiao-peng |
| Institution: | Fu Jing-li1,2,Zeng Yan3,Long Ze-ling1,Tang Xiao-peng1 1Second Xiangya Hospital/Institute of Hepatology,Central South University,Changsha 410011,Hunan Province,China,2Department of Infection,First Hospital of Zhuzhou City,Zhuzhou 412000,3Affiliated Hospital,Hunan Institute of Occupational Health,Changsha 410007 |
| Abstract: | BACKGROUND:Some traditional Chinese medicine can promote the differentiation and growth of human hematopoietic stem cells.OBJECTIVE:To observe the promotion effect of angelica sinensis(oliv.) diels and lycium chinense mill preparation for the differentiation of human umbilical cord blood stem cells(HUCBSCs) into hepatocytes in vitro and in vivo.METHODS:HUCBSCs were separated and cultured in different doses of angelica sinensis(oliv.) diels and lycium chinense mill preparation to screen the suitable dosage f... |
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