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| 人骨软骨组织体外冷冻保存的效果 | |
| 引用本文： | 亓建洪,赵建莉,张延明,宋洪强,葛孚章,张明. 人骨软骨组织体外冷冻保存的效果[J]. 中国组织工程研究与临床康复, 2011, 15(46): 8579-8582. DOI: 10.3969/j.issn.1673-8225.2011.46.007 |
| 作者姓名： | 亓建洪 赵建莉 张延明 宋洪强 葛孚章 张明 |
| 作者单位： | 1. 泰山医学院运动医学研究所,山东省泰安市,271016 2. 泰山医学院运动医学研究所,山东省泰安市271016;山东省千佛山医院关节中心,山东省济南市250014 3. 山东省千佛山医院关节中心,山东省济南市,250014 |
| 摘 要： | 背景:异体骨软骨移植是治疗关节软骨缺损的有效方法,然而由于移植物体外有效保存时间短,限制了临床应用以及移植物的利用效率。目的:观察梯度降温冷冻保存同种异体关节软骨的保存效果。方法:将关节软骨块经体积分数10%二甲基亚砜预处理后,应用程序冷冻仪以-1℃/min行梯度降温至-4℃,-10℃,-20℃,-40℃各30min,最后至-80℃冷冻保存。结果与结论:保存1,3,6个月及1年后检测发现,与新鲜组相比,冷冻后软骨细胞存活率、细胞活性以及浅、中、深层的蛋白多糖水平下降(P<0.05),且软骨细胞存活率、细胞活性以及浅、中、深层的蛋白多糖水平随冷冻保存时间延长而降低(P<0.05),提示冷冻保存可有效地延长骨软骨移植物存活时间。 |
| 关 键 词： | 关节软骨 骨软骨 冷冻保存 活性 蛋白多糖 存活率 |
Cryopreservation effect of human osteochondral allografts in vitro |
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| Qi Jian-hong,Zhao Jian-li,Zhang Yan-ming,Song-Hong qiang,Ge Fu-zhang,Zhang Ming. Cryopreservation effect of human osteochondral allografts in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(46): 8579-8582. DOI: 10.3969/j.issn.1673-8225.2011.46.007 | |
| Authors: | Qi Jian-hong Zhao Jian-li Zhang Yan-ming Song-Hong qiang Ge Fu-zhang Zhang Ming |
| Affiliation: | Qi Jian-hong1,Zhao Jian-li1,2,Zhang Yan-ming1,Song-Hong qiang1,Ge Fu-zhang1,Zhang Ming2 1Institute of Sports Medicine,Taishan Medical College,Taian 271016,Shandong Province,China,2Joint Center of Shandong Qianfoshan Hospital,Jinan 250014 |
| Abstract: | BACKGROUND:Cartilage allograft transplantation is one of effective methods for treatment of articular cartilage defects.However,the short preservation time of osteochondral allograft in vitro limits its clinical application and the utilization efficiency.OBJECTIVE:To study the preservation effect of articular cartilage allograft with controlled rate cryopreservation.METHODS:The osteochondral allografts were processed by 10% DMSO and then cooled with a controlled rate freezer at a speed of-1 ℃/min to-4 ℃for 30 minutes,to-10℃ for 30 minutes,to-20 ℃ for 30 minutes,and to-40℃ for 30 minutes.Finally,they were put into a-80 ℃ refrigerator for cryopreservation.RESULTS AND CONCLUSION:After cryopreservation for 1,3 and 6 months and 1 year,the survival rate and viability of chondrocytes and the proteoglycan level in the superficial,middle and deep layers of osteochondral allograft were decreased with time going(P < 0.05) and as compared with fresh group(P < 0.05) .These findings suggest that cryopreservation can effectively prolong the survival time of osteochondral allograft. |
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