| apelin-13下调caveolae可促进血管平滑肌细胞增殖 |
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| 引用本文： | 毛小环,李兰芳,高晶,杨莉,柳威,秦旭平,陈临溪. apelin-13下调caveolae可促进血管平滑肌细胞增殖[J]. 中国组织工程研究与临床康复, 2011, 15(46): 8603-8608. DOI: 10.3969/j.issn.1673-8225.2011.46.013 |
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| 作者姓名： | 毛小环 李兰芳 高晶 杨莉 柳威 秦旭平 陈临溪 |
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| 作者单位： | 1. 南华大学药物药理研究所,湖南省衡阳市421001;湖南环境生物职业技术学院,湖南省衡阳市421005
2. 南华大学药物药理研究所,湖南省衡阳市,421001 |
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| 基金项目： | 国家自然科学基金，教育部留学回国人员科研启动基金 |
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| 摘 要： | 背景:结合课题组以往研究成果,提出caveolae可能参与apelin-13促血管平滑肌细胞增殖的假设。目的:实验观察细胞膜特殊凹陷结构caveolae参与G蛋白偶联受体APJ的内源性配体apelin-13促进大鼠血管平滑肌细胞增殖的作用。方法:采用组织贴块法培养大鼠胸主动脉血管平滑肌细胞,用MTT方法观察血管平滑肌细胞增殖,Western Blotting方法观察信号蛋白表达,免疫共沉淀技术检测信号分子复合物的形成。结果与结论:①caveolae结构破坏剂β-环糊精(5mmol/L,25h)可明显增强apelin-13诱导的血管平滑肌细胞增殖。②apelin-13(0,1,2,4,8μmol/L)刺激血管平滑肌细胞,caveolin-1的表达下调,在1μmol/L时下调明显。③β-环糊精(5mmol/L)破坏caveolae后,可使apelin-13下调caveolin-1表达的作用增强。④对照组(体积分数为0.1%胎牛血清孵育)及处理组(apelin-13刺激)caveolin-1与PI3K及ERK1/2均有复合物形成,在apelin-13刺激的情况caveolin-1-PI3K复合物减少、caveolin-1-ERK1/2复合物减少,即apelin-13可能促进caveolin-1与PI3K及ERK1/2解离。结果提示细胞膜特殊凹陷结构caveolae参与apelin-13促血管平滑肌细胞增殖作用。
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| 关 键 词： | 血管平滑肌细胞 apelin-13 APJ caveolae caveolin-1 β-环糊精 PI3K ERK1/2 |
apelin-13 promotes proliferation of vascular smooth muscle cells by downregulating caveolae expression |
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| Mao Xiao-huan,Li Lan-fang,Gao Jing,Yang Li,Liu Wei,Qin Xu-ping,Chen Lin-xi. apelin-13 promotes proliferation of vascular smooth muscle cells by downregulating caveolae expression[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(46): 8603-8608. DOI: 10.3969/j.issn.1673-8225.2011.46.013 |
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| Authors: | Mao Xiao-huan Li Lan-fang Gao Jing Yang Li Liu Wei Qin Xu-ping Chen Lin-xi |
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| Affiliation: | Mao Xiao-huan1,2,Li Lan-fang1,Gao Jing1,Yang Li1,Liu Wei1,Qin Xu-ping1,Chen Lin-xi1 1Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001,Hunan Province,China,2Hunan Polytechnic College of Environment and Biology,Hengyang 421005 |
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| Abstract: | BACKGROUND:Previous results have shown that caveolae is likely to participate in apelin-13 promotion of vascular smooth muscle cell proliferation.OBJECTIVE:APJ is a G-protein coupled receptor,and its ligand is apelin peptide.Previously,we reported PI3K and ERK1/2 signal pathway mediated proliferation of vascular smooth muscle cells(VSMCs) induced by apelin-13.This study is to observe caveolae involved in rat VSMCs proliferation induced by apelin-13 and to determine whether caveolin-1 protein binds to signal... |
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