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| MCF7细胞中肿瘤干细胞的增殖特征 | |
| 引用本文： | 黄名威,陆云飞. MCF7细胞中肿瘤干细胞的增殖特征[J]. 中国组织工程研究与临床康复, 2011, 15(45): 8404-8407. DOI: 10.3969/j.issn.1673-8225.2011.45.010 |
| 作者姓名： | 黄名威 陆云飞 |
| 作者单位： | 1. 广西医科大学附属肿瘤医院,广西壮族自治区南宁市,530021 2. 广西医科大学第一附属医院,广西壮族自治区南宁市,530021 |
| 基金项目： | 广西研究生教育创新计划资助项目(2008105981002D33)~~ |
| 摘 要： | 背景:肿瘤干细胞学说认为,肿瘤干细胞是肿瘤不断增殖的根源,并与肿瘤的浸润转移、耐药现象密切相关。目的:在单细胞水平研究体外传代培养的MCF7细胞中肿瘤干细胞含量变化规律,认识肿瘤干细胞在体外培养环境下的增殖特点。方法:利用单细胞分离种植-成瘤性克隆方法对传代培养后不同时间点MCF7细胞中肿瘤干细胞的比例连续检测,流式细胞仪同步检测相应细胞样本中CD44+CD24-/low亚群的含量变化。结果与结论:传代后培养的MCF7细胞在不同时间点其肿瘤干细胞比例及CD44+/CD24-/low亚群比例均呈现有规律的变化。但CD44+/CD24-/low亚群变化更为显著(36.84%到81.95%),而肿瘤干细胞含量仅在小范围波动(38.54%～47.39%)。结果提示传代培养的MCF7细胞中,肿瘤干细胞具有通过调节自身增殖来保持其比例相对稳定的特点;CD44+/CD24-/low亚群并不代表或者富集MCF7细胞株中的肿瘤干细胞亚群。 |
| 关 键 词： | 肿瘤干细胞 成瘤性克隆 MCF7细胞株 增殖 CD44+/CD24-/low |
Proliferation characteristics of tumor stem cells in MCF7 cell line |
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| Huang Ming-wei,Lu Yun-fei. Proliferation characteristics of tumor stem cells in MCF7 cell line[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(45): 8404-8407. DOI: 10.3969/j.issn.1673-8225.2011.45.010 | |
| Authors: | Huang Ming-wei Lu Yun-fei |
| Affiliation: | Huang Ming-wei1,Lu Yun-fei2 1The Affiliated Tumors Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China,2the First Affiliated Hospital of Guangxi Medical University |
| Abstract: | BACKGROUND:The hypothesis of tumor stem cells posited that tumor stem cells are the root of tumor proliferation constantly and closely related to tumor biological behaviors such as infiltration,metastasis and drug-resistant.OBJECTIVE:To investigate the proliferation characteristics of tumor stem cells in MCF7 cell line in vitro by studying the tumorigenic-colony forming rate at single cell level.METHODS:Tumor stem cells proportion in MCF7 cell line was detected at series timing after passaged culture by sin... |
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