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| 京尼平苷对大鼠软骨细胞增殖的影响 | |
| 引用本文： | 谭玉林,鲍同柱,柳琴,韩钰,王艳林,周纳新,陈垦,鄢飞. 京尼平苷对大鼠软骨细胞增殖的影响[J]. 中国组织工程研究与临床康复, 2011, 15(41). DOI: 10.3969/j.issn.1673-8225.2011.41.005 |
| 作者姓名： | 谭玉林 鲍同柱 柳琴 韩钰 王艳林 周纳新 陈垦 鄢飞 |
| 作者单位： | 1. 三峡大学第一临床医学院,湖北省宜昌市,443003 2. 宜昌市中心人民医院,湖北省宜昌市,443003 3. 三峡大学分子学生物研究所,湖北省宜昌市,443003 |
| 摘 要： | 背景:京尼平苷能够促进韧带成纤维细胞的增殖和胶原的合成,促进组织的修复和韧带损伤的愈合.目的:观察京尼平苷对大鼠软骨细胞增殖的影响.方法:采用二次酶消化法获得大鼠关节软骨细胞进行传代培养,并取第4 代细胞给予0,25,50,100mmol/L 京尼平苷干预,利用MTT 比色、流式细胞仪检测细胞周期等方法检测药物干预后第4 代软骨细胞的增殖情况.结果与结论:体外培养的软骨细胞随着传代次数的增加,细胞形态由原代的多角形逐渐变为长梭形;且随着培养时间的延长及京尼平苷浓度的升高,软骨细胞增殖更为显著(P < 0.01). |
| 关 键 词： | 京尼平苷 软骨细胞 增殖 大鼠 培养 |
Effect of geniposide on proliferation of rat chondrocytes in vitro |
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| Tan Yu-lin,Bao Tong-zhu,Liu Qin,Han Yu,Wang Yan-lin,Zhou Na-xin,Chen Ken,Yan Fei. Effect of geniposide on proliferation of rat chondrocytes in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(41). DOI: 10.3969/j.issn.1673-8225.2011.41.005 | |
| Authors: | Tan Yu-lin Bao Tong-zhu Liu Qin Han Yu Wang Yan-lin Zhou Na-xin Chen Ken Yan Fei |
| Affiliation: | Tan Yu-lin1,Bao Tong-zhu2,Liu Qin2,Han Yu3,Wang Yan-lin3,Zhou Na-xin2,Chen Ken2,Yan Fei2 1The First College of Clinical Medical Science,China Three Gorges University,Yichang 443003,Hubei Province,China,2Yichang Central People's Hospital,3Institute of Molecular Biology,Three Gorges University |
| Abstract: | BACKGROUND:Studies have shown that geniposide can ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen.OBJECTIVE:To investigate the effect of geniposide on the proliferation of rat chondrocytes in vitro.METHODS:The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial.The chondrocytes of passage 4 were interfered with 0,25,50,100 mmol/L geniposide.Chonddrocyte proliferation was detected using methyl th... |
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