木瓜蛋白酶诱导膝关节骨关节炎模型兔滑膜病理变化与药物注射时间的关系

背景:木瓜蛋白酶诱导兔膝关节骨性关节炎的造模方法可获得稳定的退行性关节炎模型。目的:观察兔膝关节腔内注射木瓜蛋白酶诱导骨关节炎后,滑膜炎性病理改变与药物注射时间的关系。方法:将木瓜蛋白酶水溶液分别在实验开始的第1,4,7天分别注射入15只西兰大白兔右膝关节腔内,建立膝关节骨性关节炎兔模型,于首次注射后第2,4,6周分别观察膝关节滑膜大体及组织病理学变化,每个时间点5只。结果与结论:木瓜蛋白酶首次注射后第2周,兔膝关节滑膜炎性反应最严重,表现为滑膜细胞增生最明显,下层疏松纤维及血管组织明显增生,可见较多淋巴浆细胞浸润,第4,6周时炎性反应逐渐减轻。说明兔膝关节首次注射木瓜蛋白酶后2周时滑膜炎性反应最严重。

Relationship between synovium pathological change and injection timing in rabbits with papain-induced knee osteoarthritis

BACKGROUND:A stable degenerative knee osteroarthritis model in rabbits can be established by the induction of papain.OBJECTIVE:To explore the relationship between the pathological change of synovium and injection timing in rabbits with knee osteoarthritis induced by intraarticular injection of papain.METHODS:Papain solution was injected into the right knee joint cavity of 15 New Zealand white rabbits on the 1st,4th and 7th days to induce knee osteoarthritis in rabbits.The general and histopathologic changes of knee synovium were examined in the 2nd,4th and 6th weeks after first injection.Five rabbits were examined at each time point.RESULTS AND CONCLUSION:The synovium inflammatory reaction of rabbit knee joint was the most serious at the 2nd week after the first injection of papain,characterized by the most obvious proliferation of synovial cell,obvious proliferation of vascular tissue and substratum porosity fiber vascular tissue,visible infiltration of many lymphoplasmacytoid cells.The inflammatory reaction relieved gradually in the 4th and 6th weeks.These results illustrate that the synovium inflammatory reaction of knee joint in rabbits reach its maximum effect in the 2nd week after the first injection of papain.