选择性环氧合酶-2抑制剂NS-398对食管癌细胞株EC9706的生长抑制作用

目的:探讨NS-398对人食管癌细胞株EC9706的生长抑制作用。方法:采用3H-TdR掺入法检测不同浓度(0、10、20、50、100μmol/L)和作用24、48、72、96h的NS-398对细胞的增殖抑制效应;采用流式细胞术(FCM)及DNA片段分析法检测NS-398诱导的细胞凋亡情况。结果:EC9706经不同浓度的NS-398处理后,实验组3H-TdR掺入量明显减少,具有时间和剂量依赖关系,与对照组比较差异显著(P<0.05或P<0.001)。FCM检测发现实验组在G1期前出现亚倍体凋亡峰,细胞凋亡率达(45.23±1.08)%,并出现典型的细胞凋亡梯带;而对照组无凋亡峰出现,细胞凋亡率为(2.14±0.28)%,两组比较有显著性差异(P<0.001)。结论:NS398对食管癌细胞株EC9706细胞具有增殖抑制作用和诱导细胞凋亡作用。

Growth Inhibitory Effects of a Selective Cyclooxygenase-2 Inhibitor NS-398 on Esophageal Carcinoma Cell Line EC9706

Objective To study the growth Inhibitory effects of a selective cyclooxygenase-2 (COX-2)inhibitor NS-398 on esophageal carcinoma cell line EC9706. Methods The esophageal carcinoma cell line EC9706, expressing COX-2 constitutively, was incubated with NS-398 at 0,10,20,50,100 μmol/L for 24 h,48 h, 72 h and 96 h, respectively.The antiproliferation effect of NS-398 was measured by 3H-TdR incorporation,meanwhile the cell apoptosis was determined by flow cytometry(FCM) and DNA fragmentation analysis.Results The growth of EC9706 cells could be inhibited by NS 398 in a dose-and time-dependent manner.FCM analysis demonstrated a high sub-G_1 cell peak in NS-398 group, agarose electrophroesis showed marked apoptosis ladder pattern, but no apoptosis peak in control group was observed.The apoptosis percentage between NS-398 group (45.23±1.08)% and control group (2.14±0.28)% was significantiy different(P<0.001). Conclusion The selective COX-2 inhibitor NS-398 could suppress cell growth and increase apoptosis in esophageal Carcinoma cell line EC9706.