| 人白细胞介素6表达的核因子蛋白在大肠杆菌中的表达与鉴定 |
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| 引用本文： | 人白细胞介素6表达的核因子蛋白在大肠杆菌中的表达与鉴定[J]. 中国药科大学学报, 1998, (5): 77-80. |
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| 作者姓名： | 朱敏生 刘定干 |
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| 作者单位： | 南京军区军事医学研究所，中国科学院上海生物化学研究所 |
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| 摘 要： | 将ＮＦ－ＩＬ６基因与谷胱甘肽转移酶（ＧＳＴ）基因融合并成功地表达出具有生物活性的ＮＦ－ＩＬ６，经ＧｅｌＲｅｔａｒｄａｔｉｏｎ分析证实该重组蛋白能与其ＤＮＡ识别元件结合。实验还发现，虽然重组蛋白的表达率较低，但能形成不溶性包含体
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| 关 键 词： | NF－IL6 融合表达 大肠杆菌 鉴定 |
Recombinant Expression of Human NF IL6 in E coli and Its Bioactivity Determination |
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| Recombinant Expression of Human NF IL6 in E coli and Its Bioactivity Determination[J]. Journal of China Pharmaceutical University, 1998, (5): 77-80. |
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| Authors: | Zhu Minsheng Liu Dinggan Xu Xiangyu Shen Yue Li Zaiping Nanjing Military Institute of Medical Science Nanjing |
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| Affiliation: | Zhu Minsheng,Liu Dinggan 1,Xu Xiangyu,Shen Yue,Li Zaiping 1 Nanjing Military Institute of Medical Science,Nanjing 210002, 1Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031 |
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| Abstract: | NF IL6, a nuclear factor for interleukine 6 expression, is a member of C/EBP family It plays an important role in gene regulation of inflammation, differenciation, acute phase reaction, forming of cytokine net, tumoregenesis and apoptosis In this report, we fused GST gene to NF IL6 gene and recombinantly expressed it in E coli The produced protein can bind to NF IL6 binding motif determined with Gel Retardation Assay In addition, we found that recombinant protein formed inclusion body , although the expression level was low |
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| Keywords: | NF IL6 Fusion expression E coli Bioactivity determination |
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