3,4,5-三羟基苯甲酸对H_(22)肝癌小鼠实体瘤的抑制作用及其机制

目的：观察3，4，5-三羟基苯甲酸（TBA）对H₂₂肝癌小鼠实体瘤的抑制作用及其对荷瘤小鼠生存时间和体内抗氧化能力的影响。方法：建立移植性H₂₂肝癌细胞荷瘤小鼠模型，造模成功小鼠随机分为空白对照组，阳性药对照组，TBA低、中、高剂量组；每组10只。对照组0.5% 羧甲基纤维素钠溶液0.2 mL·10 g^-1灌胃给药,阳性药对照组CTX 20 mg·kg^-1腹腔注射，TBA低、中、高剂量组TBA溶液0.13、0.25 和0.50 g·kg^-1灌胃给药，每天固定时间给药1次，连续给药10 d。检测各组小鼠实体瘤质量、小鼠生存时间、血清超氧化物歧化酶（SOD）活性、丙二醛（MDA）和还原型谷胱甘肽（GSH）水平。结果：与空白对照组比较，TBA中剂量组和CTX阳性药对照组小鼠平均瘤质量下降（P<0.05），其他各组间比较差异无显著性；与空白对照组比较，TBA中剂量组和CTX阳性药对照组小鼠平均生存时间延长（P<0.05），其他各组间比较差异无显著性；与空白对照组比较，TBA中、高剂量组小鼠血清SOD活性升高（P<0.05），中剂量组血清MDA 水平降低（P<0.05），低、中、高剂量组血清GSH升高（P<0.05），其他各组间比较差异无显著性。结论：TBA灌胃给药可以不同程度抑制H₂₂ 肿瘤的生长，延长荷瘤小鼠生存时间；TBA可以清除荷瘤小鼠体内自由基，上调机体的氧化应激状态。

Inhibitory effects of 3,4,5-TBA on H22 tumor bearing mice and mechanism

Objective To observe the inhibitory effect of 3, 4, 5-hydroxy benzoic acid (TBA) on H_(22) transplanted tumor in mice and its influence in survival time and antioxidant capacity in vivo of H_(22) tumor bearing mice .Methods H_(22) tumor bearing mouse models were established.The H_(22) tumor bearing mice were randomly divided into control group, positive control group, TBA low dose group,group.There were 10 mice in each group. 0.5％ sodium carboxymethyl cellulose solution 2 mL·10 g~(-1) was administered by oral in control group.CTX 20 mg·kg~(-1) was injected intraperitoneally in positive control group.TBA was administered by oral in TBA low (0.13 g·kg~(-1)), middle (0.25 g·kg~(-1)) and high (0.50 g·kg~(-1)) TBA middle dose group, and TBA high dose dose groups.The H_(22) tumor bearing mice were administered once a day at fixed time and lasted for 10 d.Then, the tumor weight, the survival time, and the SOD activity, MDA and GSH levels in serum in each group were detected.Results Compared with control group, the average tumor weights were reduced in TBA middle dose group and CTX group (P<0.05), there was no significant difference between other groups;compared with control group, the mean survival time in TBA middle dose group and CTX group were longer (P<0.05), there was no significant difference between other groups;compared with control group, the SOD activities in TBA middle and high dose groups were significantly increased (P < 0.05);the MDA level in TBA middle dose group was significantly decreased (P<0.05);the GSH levels were in TBA low, middle, high dose groups were significantly increased (P<0.05) .Conclusion TBA could inhibit the growth of H_(22) tumor and prolong the survival time of tumor bearing mice by oral administration.TBA could clear the free radicals in tumor bearing mice and up-regulate the oxidative stress status of body.