食源性肥胖大鼠2型糖尿病模型的建立

背景：高脂饮食加小剂量链脲佐菌素是目前国内最常用的2型糖尿病大鼠造模方式,但高脂饮食仅能诱导50%大鼠发生胰岛素抵抗,因此还需寻找更理想的2型糖尿病大鼠建模方法。目的：选择食源性肥胖大鼠进行小剂量链脲佐菌素腹腔注射,建立更理想的2型糖尿病动物模型。方法：将SD大鼠随机分成对照组和高脂饮食组。4周后,据大鼠体质量将高脂饮食组分为食源性肥胖组和食源性肥胖抵抗组。8周后,所有大鼠均给予小剂量链脲佐菌素(30 mg/kg)腹腔注射,将注射10 d后空腹血糖> 7.8 mmol/L且稳定2周以上者纳为2型糖尿病大鼠模型。检测各组大鼠的空腹血糖、血脂、胰岛素;评价各组大鼠的胰岛素抵抗程度;比较各组大鼠的2型糖尿病成模率。结果与结论：与对照组及食源性肥胖抵抗组相比,食源性肥胖组大鼠出现明显的体质量增加、高血脂、高胰岛素血症及胰岛素抵抗(P < 0.01)。注射链脲佐菌素后食源性肥胖组2型糖尿病成模率高达100%,食源性肥胖抵抗组成模率仅为12.5%。由结果可知选择食源性肥胖组大鼠作为2型糖尿病造模对象,是2型糖尿病造模方法的成功改良。

Establishment of diet-induced type 2 diabetes mellitus rat models

BACKGROUND: Fat-fed plus small dose of streptozotocin are widely used to induce rat type 2 diabetes mellitus (T2DM) models in china, while only half of fat-fed rats develop insulin resistance. Thus, it is necessary to explore ideal methods for T2DM model establishment.OBJECTIVE: To explore a novel animal model for T2DM by selecting the diet-induced obese (DIO) rats plus streptozotocin injection. METHODS: Sprague Dawley rats were randomly divided into the control and high fat diet groups. Four weeks later, rats of HF diet group were divided into DIO group and diet-induced obesity resistance (DR) group by body weight. Eight weeks later, rats in all groups were injected with streptozotocin intraperitoneal (30 mg/kg). Rats with fasting blood glucose >7.8mmol/L and maintained for 2 weeks were enrolled in T2DM. The levels of fasting blood glucose, total cholesterol and triglyceride, serum insulin, insulin sensitivity were determined. Diabetic successful rate was compared among groups. RESULT AND CONCLUSION: Compared with the control and DR group, the body weight, total cholesterol, triglyceride, serum insulin and insulin resistance increased significantly in DIO group (P < 0.01). Diabetic successful rate in DIO group reached to 100%. It is a successful improvement to establish rat models of T2DM by selecting DIO rats combined with STZ injection.