蒙药寒水石化灰小剂药效学研究

目的：研究寒水石化灰小剂的抗癌活性、免疫调节作用及抗菌活性。方法：利用血清药理学方法制备寒水石化灰小剂的含药血清，MTT 法观察含药血清对体外培养的S180 腹水瘤细胞和人食道癌细胞增殖率的影响及对小鼠脾淋巴细胞转化的影响，牛津杯法测定寒水石化灰小剂体外的抗菌活性。结果：寒水石化灰小剂含药血清抑制S180 和人食道癌细胞增殖，对人食道癌细胞的抑制与5-氟尿嘧啶（5-Fu）有协同作用；诱导小鼠脾淋巴细胞转化率降低，但对5-Fu 所致的小鼠脾细胞转化率降低有抑制作用；汤剂浓度在0.25 g·mL-1 以上表现有抗菌活性。结论: 体外实验表明，寒水石化灰小剂能抑制S180 腹水瘤细胞和人食道癌细胞增殖，显著逆转免疫抑制剂体外诱导的小鼠脾细胞转化率降低，抗菌活性尚待进一步研究。

Pharmacodynamic Study on Mongolian Medicine Calcitum Ashing Agent

This study was aimed to preliminary explore the anticancer activity, immunomodulatory effects and antimicrobial activity of Calcitum ashing agent. Serum pharmacological method was used to prepare the serum containing Calcitum ashing agent. MTT method was used to observe the cell proliferation rate of S180 ascites tumor cells and human esophageal cancer cells cultured with the serum containing Calcitum ashing agent in vitro. In addition, this method was also used to observe the mouse spleen lymphocyte transformation rate. The Oxford Cup method was used to determine the antibacterial activity on Calcitum ashing agent in vitro. The results showed that serum containing Calcitum ashing agent inhibited the cell proliferation rate of S180 and esophageal cancer cells. Meanwhile, it had a synergistic effect with 5-fluorouracil (5-FU) on the inhibition of human esophageal cancer cells. The serum containing Calcitum ashing agent induced the reduction in the mice spleen lymphocytes transformation rate. But it inhibited the reduction in the spleen lymphocytes transformation rate induced by 5-FU. The decoction with the concentration higher than 0.25 g·mL-1 had significantly inhibited the proliferation of bacteria. It was concluded that Calcitum ashing agent can inhibit the cell proliferation of S180 ascites tumor cells and human esophageal cancer cell in vitro. It can significantly reverse the reduction in the mice spleen lymphocytes transformation rate induced by immunodepressant. However, the antibacterial activity still requires further study.