miR-155在淋巴瘤Raji细胞辐射抵抗中作用的研究

目的 研究miR-155在淋巴瘤Raji细胞辐射抵抗中的作用及其机制。方法 实时定量聚合酶链反应检测Raji细胞miR-155的表达,CCK-8法检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测PTEN和p-AKT的表达。结果 miR-155 RNA和p-AKT蛋白在Raji细胞中表达较高,而pten基因mRNA和蛋白质表达水平极低,单纯4.0 Gy照射48 h后Raji细胞凋亡率较低;miR-155 siRNA不仅使miR-155 RNA和p-AKT表达水平明显下降,而且pten基因mRNA和蛋白质水平显著增高,同时细胞显示出增殖抑制作用,显著增加Raji细胞对该辐射剂量敏感性,siRNA+射线照射组细胞凋亡率为(36.78±1.35)%,高于单纯照射组(t=12.572,P<0.05)。结论 miR-155 表达对Raji细胞辐射敏感性具有重要影响,其机制与PTEN/PI3K/AKT信号途径激活有关。

Role of miR-155 in radioresistance of lymphoma Raji cells

Objective To investigate the role of miR-155 in radio-resistance of lymphoma cell line Raji cells.Methods The expression of miR-155 was detected by quantitative real-time PCR, cell growth was detected by CCK-8 assay, cell apoptosis was detected by flow cytometry, and the protein expressions of PTEN and p-AKT were detected by Western blot. Results The Raji cells had high levels of miR-155 RNA and p-AKT protein had a very low level of PTEN protein. Cell apoptosis was induced by 4.0 Gy X-ray irradiation after 48 h. The transfection of cells with miR-155 siRNA not only silenced miR-155, but also significantly decreased p-AKT level, increased the expression of pten gene and protein level, inhibited cell proliferation, and increased the radiation sensitivity of Raji cell, the apoptosis rate of siRNA+ irradiation group(36.78%±1.35%) was higher than that of irradiation group(t=12.572,P<0.05).Conclusions High levels of miR-155 was involved in radiation resistance of lymphoma Raji cells and this activity is likely modulated by the PTEN/PI3K/AKT signaling pathway.