| 日本血吸虫重组32kD抗原的纯化和诊断应用 |
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| 引用本文： | 舒新华. 日本血吸虫重组32kD抗原的纯化和诊断应用[J]. 湖南医科大学学报, 1998, 23(4): 331-334 |
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| 作者姓名： | 舒新华 |
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| 摘 要： | 用ＳＤＳ－ＰＡＧＥ结合电渗洗脱的方法分离，纯化日本血吸虫重组３２ｋＤ抗原；用酶联免疫印迹技术分析纯化抗原的免疫活性。用纯化抗原，日本血吸虫成虫抗原和可溶性虫卵抗原分别检测慢性血吸虫病人肺吸虫病，肝吸虫病人，钩虫和健康人血清抗体。
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| 关 键 词： | 日本血吸虫 日本血吸虫病 纯化 rSj32 |
Schistosoma japonicum: purification and diagnostic application of recombinant 32 kD antigen] |
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| X Shu,J Zhou,C Cai,X Zeng,Q Zeng,X Yi. Schistosoma japonicum: purification and diagnostic application of recombinant 32 kD antigen][J]. Bulletin of Hunan Medical University, 1998, 23(4): 331-334 |
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| Authors: | X Shu J Zhou C Cai X Zeng Q Zeng X Yi |
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| Affiliation: | Department of Parasitology, Hunan Medical University, Changsha 410078. |
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| Abstract: | Separation and purification of rSj32 were carried out with SDS-PAGE and electroelution technique. The purified rSj32, AWA and SEA were used to examine sera of patients with schistosomiasis, healthy individuals and patients with other parasitic diseases by ELISA. The results showed that the molecule weight of rSj32 was 37 kD. The sensitivity and specificity of rSj32 as a coating antigen in ELISA were as good as those of AWA and SEA. It is suggested that rSj32 antigen is a potential antigen for immunodiagnosis of schistosomiasis japonica. |
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