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| 海绵分离富集尿内17—酮类固醇和17—羟皮质类固醇的测定 | |
| 引用本文： | 荆照政 傅兴平. 海绵分离富集尿内17—酮类固醇和17—羟皮质类固醇的测定[J]. 湖南医科大学学报, 1998, 23(3): 314-316 |
| 作者姓名： | 荆照政 傅兴平 |
| 作者单位： | [1]湖南医科大学附属湘雅医院中心实验室 [2]平江县中医院检验科 |
| 摘 要： | 用聚氨脂海绵对人尿内１７－酮类固醇（１７－ＫＳ）和１７－羟皮质类固醇（１７－ＯＨＣＳ）进行分离和富集。用乙醇解吸附，分别用间二硝基苯和盐酸苯肼显色，分光光度法测定。本法标准差为±０．０３２，检出限为０．０１μｇ·ｍｌ＾－１，回收率为９９．０％￣１０１．０％，变异系数小于６．２３％。 |
| 关 键 词： | 17－酮类固醇 聚氨脂海绵 尿 17羟皮质类固醇 |
Detection of 17-ketosteroid and 17-hydroxycorticosteroid in urine after separation and collection by foam rubber] |
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| Z Jing,X Shi,X Fu. Detection of 17-ketosteroid and 17-hydroxycorticosteroid in urine after separation and collection by foam rubber][J]. Bulletin of Hunan Medical University, 1998, 23(3): 314-316 | |
| Authors: | Z Jing X Shi X Fu |
| Affiliation: | Central Laboratory, Xiangya Hospital, Hunan Medical University, Changsha. |
| Abstract: | Using foam rubber 17-ketosteroid (17-KS) and 17-hydroxycorticosteroid (17-OHCS) were separated and collected in urine. 17-KS and 17-OHCS were eluted down from the foam rubber with alcohol, colour reaction can be respectively produced by m-dinitrobenzene and phenyhydrazine hydrocholoride, then both of them were detected by spectro-photometry. The standard deviation of this method was +/- 0.032, and its minimum detectable concentration was 0.01 microgram.ml-1. The recovery rate of 17-KS and 17-OHCS estimation was from 99.0% to 101.0%, with co-efficients of variation less than 6.23%. |
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