Safflor yellow B suppresses pheochromocytoma cell (PC12) injury induced by oxidative stress via antioxidant systemand Bcl-2 /Bax pathway

Our previous study demonstrates that SYB produces a neuroprotective effect in vivo. In the present study, we investigated the protective effect of safflor yellow B (SYB) on the acute oxidative injury induced by H₂O₂ and mechanisms in PC12 cells. H₂O₂ was used to mimic in vitro model of the oxidative injury and to induce apoptosis in PC12 cells. The cells were pretreated with the different concentrations of SYB. The cell viability, lactate dehydrogenase (LDH) release, malondialdehyde (MDA), and superoxide anion levels, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured. Caspase 3 activity, Bcl-2 and Bax expressions were also observed. The results showed that exposure of the cells to H₂O₂ significantly decreased the cell viability, SOD and GSH-Px activities and Bcl-2 expression, and increased LDH release, superoxide anion and MDA generations, caspase 3 activity and Bax expressions. Pretreatment of the cells with SYB was able to remarkably antagonize the H₂O₂-induced changes in dose-dependent way. These suggest that SYB is able to protect PC12 cells from H₂O₂-induced injury and apoptosis via antioxidant and anti-apoptotic mechanisms.