| 高效液相色谱法测定红金耳环中马兜铃酸A的含量 |
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| 引用本文： | 钟燕珠,柴婷婷,李知源,邓超明. 高效液相色谱法测定红金耳环中马兜铃酸A的含量[J]. 现代医药卫生, 2009, 25(15): 2245-2246 |
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| 作者姓名： | 钟燕珠 柴婷婷 李知源 邓超明 |
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| 作者单位： | 钟燕珠(广东省中医院药学部,广东,广州,510120);柴婷婷,李知源,邓超明(广州中医药大学中药学院,广东,广州,510405) |
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| 摘 要： | 目的：对红金耳环中马兜铃酸A进行定量分析。方法：采用高效液相色谱（HPLC）法测定马兜铃酸A的含量，色谱柱：Kromasi C18（250mm×4．6mm，5μm），流动相甲醇-水-冰醋酸（65：34：1），流速1．0ml·min^-1，检测波长310nm，柱温25℃。结果：马兜铃酸A在0．16—0．80μg范围内呈良好的线性关系，r为0．9995，平均加样回收率为98．97％，RSD为2．10％。红金耳环中马兜铃酸A的含量为0．0012％。结论：该方法简便，重现性好，可用于测定红金耳环中马兜铃酸A的含量。
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| 关 键 词： | 红金耳环 马兜铃酸A 高效液相色谱法 |
Content determination of aristolochic acid A in Asarum petelotii O.C. Schmidt by HPLC |
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| Affiliation: | ZHONG Yan-zhu, CAI Ting-ting, LI Zhi-yuan, et al. (1.Department of Pharmacy , Guangdong Provincial Hospital of Chinese Medicine , Guangzhou 510120 , China ; 2.School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China) |
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| Abstract: | Objective：To analyse aristolochic acid A in Asarum petelotii O.C. Schmidt in order to guide use in clinic. Methods：The content of aristolochinc acid A in Asarum petelotii O.C. Schmidt was determined by HPLC on Kromasi C18（250 mm×4.6 mm,5 μm） with the mobile phase consisted of methanol-water-glacial acetic acid （65：34：1）. The flow rate was 1.0 ml. min^-1.The detection wavelength was 310 nm. Column temperature was set at 25 ℃.Results：The method of HPLC had a good linearity relationship in the range of 0.16-0.80 μg （r=0.999 5）.The average recovery was 98.97% （RSD=2.10%）. Conclusion：HPLC used in content determination of aristolochinc acid A is precise and reproducible,which can be used for precisely analysing the quantity of aristolochic acid A in Asarum petelotii O.C. Schmidt. |
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| Keywords: | Asarum petelotii O.C. Schmidt Aristolochic acid A HPLC |
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