醋酸地塞米松PEG-PLGA纳米粒的制备及表征

目的 研究醋酸地塞米松PEG-PLGA纳米粒的制备工艺及影响因素,并优化冻干粉针工艺.方法 采用乳化/溶剂蒸发法制备醋酸地塞米松PEG-PLGA纳米粒,并考察其粒径分布、包封率、载药量等;采用单因素试验筛选出合适的冻干保护剂.结果 醋酸地塞米松PEG-PLGA纳米粒的粒径为91.43±1.00 nm,Zeta电位为-22.73 ±0.57 mv,包封率为88.78％ ±2.10％,载药量为4.95％-±0.23％;10％蔗糖作为冻干保护剂的效果最好,冻于复溶后粒径约117.27 nm.结论 所用制备工艺简单易行,可用于制备醋酸地塞米松PEG-PLGA纳米粒.

Preparation and characterization of Dexamethasone acetate loaded PEG-PLGA nanoparticles

OBJECTIVE To prepare Dexamethasone acetate loaded PEG-PLGA nanoparticles and explore the influence factors and to optimize the supporting materials for fieeze-dried injections.METHODS The emulsion/solvent evaporation method was employed to prepare Dexamethasone acetate loaded PEG-PLGA nanoparticles.The characteristics were studied on the terms of particle size distribution,entrapment efficiency and drug loading.The supporting materials were determined by single factor optimization.RESULTS The average nanoparticle size was 91.43 ± 1.00 nm;entrapment efficiency was 88.78％ ± 2.10％;drug loading was 4.95％ ± 0.23％.It showed that 10％ of sucrose was the best was supporting material.The particle size of resuspended NPs was approximately 117.27 nm.CONCLUSION The preparation process is simple,which can be used to prepare Dexamethasone acetate loaded PEG-PLGA nanoparticles.