肾清饮对腺嘌呤致大鼠肾间质纤维化的防治作用

目的观察肾清饮对大鼠肾问质纤维化的影响，并探讨其机制。方法将56只大鼠随机分为正常对照组（C组）、模型组（M组）和药物干预组，药物干预组包括肾清饮预防组（S组）、肾清饮低剂量组（SL组）、肾清饮高剂量组（SH组）、苯那普利组（B组）、肾清饮＋苯那普利组（S＋B组），每组8只。对M组和药物干预组大鼠采用腺嘌呤灌胃法建立肾间质纤维化动物模型。M组给予腺嘌呤灌胃，第4～6周给予蒸馏水灌胃；S组前3周同时给予腺嘌呤及肾清饮（0．4ml／d）灌胃，每毫升肾清饮含生药2．4g，第4～6周给予肾清饮（0．4ml／d）灌胃；SL组和SH组前3周给予腺嘌呤灌胃，第4～6周给予肾清饮灌胃，剂量分别为0．4ml／d和1．2ml／d；B组前3周给予腺嘌呤灌胃，第4～6周给予盐酸苯那普利灌胃，剂量为10mg·kg^-1·d^-1；S＋B组前3周给予腺嘌呤灌胃，第4～6周同时给予盐酸贝那普利（10mg·kg^-1·d^-1）及肾清饮（0．4ml／d）灌胃。比较各组大鼠血肌酐（SCr）、尿素氮（BUN）、24h尿蛋白定量、血浆内皮素1（ET-1）水平、肾组织病理学表现、问质纤维化指数评分及肾组织Ⅰ型胶原、Ⅲ型胶原和转化生长因子β1（TGF-β1）mRNA表达。结果与C组相比，M组SCr、BUN、24h尿蛋白定量和血浆ET-1水平及肾组织Ⅰ型胶原、Ⅲ型胶原和转化生长因子B1（TGF-β1）mRNA表达显著升高（P〈0．01）；与M组相比，各药物干预组SCr、BUN、24h尿蛋白定量和血浆ET-1水平及肾组织Ⅰ型胶原、Ⅲ型胶原和TGF-β1 mRNA表达显著降低（P〈0．01），肾间质纤维化程度减轻（P〈0．05）；各药物干预组之间相比，s组SCr、BUN、24h尿蛋白定量和血浆ET-1水平及肾组织Ⅰ型胶原、Ⅲ型胶原和TGF-131mRNA表达降低最显著（P〈0．01）。结论肾清饮可延缓肾间质纤维化，减少尿蛋白，改善肾功能。

Effect of Shenqingyin on adenine-induced renal interstitial fibrosis in rats

Objective To investigate the effect of Shenqingyin on adenine-induced renal imerstitial fibrosis in rats. Methods Fifty-six rats were divided into 7 groups： group C, group M, group S, group SL, group SH,group B and group S＋ B,8 in each group. The models of renal interstitial fibrosis were induced by intragastric administration of adenine in rats. In group M, the rats were subjected to intragastric administration of adenine for 3 weeks, followed by distilled water in next 4th-6th weeks, In group S, the rats were subjeered to intragastrie administration of adenine and Shenqingyin （0. 4 ml/d） for 3 weeks,followed by Shenyingyin （0. 4 ml/d） in next 4th-6th weeks, In group SL and SH,Shenqingyin （0. 4 ml/d or 1.2 ml/d ） was given at the 4th-6th week. In group B, benazepril （10 mg·kg^-1·d^-1）was given at the 4th - 6th week. In group S＋ B, both benazepril（10 mg·kg^-1·d^-1）and Shenqingyin （0. 4 ml/d） were given at the 4th-6th week. Serum ereatinine （SCr）,blood ttrea nitrogen （BUN）,endothelin-1 （ET-1）,24-h urine protein, the degree of renal interstitial fihmsis and the renal rnRNA expression of collagen Ⅰ, collagenⅢ and trarfrom-fing growth factor-β1（TGF-β1） were compared. Results As compared with group C, SCr, BUN, ET-1,24-h urine protein,the degree of renal interstitial fibrosis and the mRNA expression of collagenI, collagen Ⅲ and TGF-β1 were increased （P〈0. 01 ） in group M. As compared with group M, above mentioned indexes were decreased in Shenqingyin intervention groups （P〈0. 01） ,and most signficantly in group S （P〈0. 01）. Conclusions Shenqingyin may slow down the progression of renal interstitial fibrosis, irnprovet kidney function, and reduce urine protein.