非小细胞肺癌动态增强磁共振成像参数与血清PCNA、Survivin及VEGF表达的相关性研究

目的:研究分析非小细胞肺癌(NSCLC)患者动态增强磁共振成像(DCE-MRI)定量参数与血清增殖细胞核抗原(PCNA)、凋亡抑制蛋白(Survivin)和血管内皮生长因子(VEGF)表达水平的相关性.方法:选取医院收治的137例NSCLC患者纳入观察组,另选同期进行体检的137名健康者纳入健康对照组.采用磁共振扫描仪...

A relevant research between DCE-MRI parameters and expressions of PCNA,survivin and VEGF in NSCLC

Objective:To research and analyze the correlations between quantitative parameters of dynamic contrastenhanced magnetic resonance imaging(DCE-MRI)and the expressions of proliferating cell nuclear antigen(PCNA),apoptotic inhibitor protein(Survivin),vascular endothelial growth factor(VEGF)in serum of patients with non-small cell lung cancer(NSCLC).Methods:137 patients with NSCLC who admitted to hospital were divided into observation group,and 137 healthy people who underwent physical examination in the same period were divided into healthy control group.The magnetic resonance(MR)scanner was adopted to detect transfer constant of volume(K^trans),speed constant(K_ep)and the volume ratio between extravascular and extracellular interval(V_e)of DCE-MRI quantitative parameters.The enzyme linked immunosorbent assay(ELISA)was adopted to detect the levels of serum PCNA,Survivin and VEGF of two groups,and the K^trans,K_ep and V_e of DCE-MRI were compared and analyzed.And the relations of the expression levels of serum,K^trans,K_ep and V_e and clinicopathologic features of observation group were analyzed.The correlations of serum levels of PCNA,Survivin and VEGF and the values of K^trans,K_ep and V_e of two groups were analyzed by Pearson correlation analysis method.Results:The levels of serum PCNA,Survivin and VEGF of observation group were significantly higher than those of healthy control group(t=50.025,t=8.724,t=16.330,P<0.05),respectively.And the values of K^trans,K_ep and V_e of observation group were significantly higher than those of healthy control group(t=8.258,t=7.937,t=14.046,P<0.05).In the observation group,the expression levels of serum PCNA,Survivin and VEGF of minor differentiation,TNM III stage and lymphatic metastasis were significantly higher than those of high-middle differentiation,TNM I-II stage and nonlymphatic metastasis(t_PCNA=4.192,t=4.327,t=5.857,t_Survivin=3.197,t=3.428,t=5.140,t_VEGF=4.915,t=4.724,t=4.485,P<0.05),respectively.The values of K^trans,K_ep and V_e of minor differentiation,TNM III stage and lymphatic metastasis were significantly higher than those of high-middle differentiation,TNM I-II stage and non-lymphatic metastasis(t_K^trans=5.159,t=5.514,t=4.841,tK_ep=6.043,t=5.776,t=5.690,tV_e=5.265,t=6.076,t=6.783,P<0.05),respectively.In observation group,the expression levels of serum PCNA and Survivin were positive correlation with the values of K^trans and K_ep(r_PCNA=0.435,r=0.402,r_Survivin=0.382,r=0.393,P<0.05),and the expression level of VEGF was positive correlation with the values of K^trans,K_ep and V_e(r=0.503,r=0.405,r=0.316,P<0.05),respectively.Conclusion:There are a certain of correlations between DCE-MRI quantitative parameters and the serum PCNA,Survivin and VEGF in NSCLC patients,and the DCE-MRI quantitative parameters and the detections of serum PCNA,Survivin and VEGF have a certain of reference value in judging the state of disease and assessing the prognosis for patients.