川芎嗪对缺氧大鼠血浆CGRP水平及CGRP,PDGF,bFGF在肺内表达的影响

 目的:研究缺氧性肺动脉高压(HPH)大鼠血浆降钙素基因相关肽(SCGRP)水平,CGRP及碱性成纤维细胞生长因子(bFGF)、血小板源性生长因子(PDGF)在肺内表达和川芎嗪(ligustrazine,Li)对其影响。方法:建立SD大鼠常压HPH模型;用酶联免疫检测技术测定SCGRP水平;用免疫组化染色检测CGRP,PDGF,bFGF在肺内表达水平。结果:缺氧(H)组大鼠SC-GRP较正常对照(N)组明显下降,P<0.01;缺氧加川芎嗪(H+Li)组SCGRP水平较H组无显著性差异,P>0.05;免疫组化染色显示H组CGRP,bFGF,PDGF的肺内表达均显著增强,而H+Li组bFGF,PDGF表达水平与H组比较明显降低,但CGRP表达不受Li影响。结论:HPH的形成与SCGRP下降和肺内PDGF,bFGF表达增强有关;Li抑制缺氧大鼠肺内PDGF和bFGF表达是其防治HPH的机制之一。

Effects of ligustrazine on serum CGRP and on the expression of CGRP,PDGF and bFGF in lung of hypoxic rats

OBJECTIVE:To study the effects of ligustrazine on the SCGRP and on the expression of CGRP,PDGF and bFGF in the lung of CHPH rats.METHOD:A stable rat modle of CHPH was established.The serum CGRP levels were mesured by enzyme immunoassay.The expression of CGRP,PDGF and bFGF in lumg were observed by immunohistochemical method.RESULTS:①The SCGRP level of H group was significantly lower than that of N group (P<0.01) but no difference between the H+Li group and H group (P>0.05).②The expression of CGRP,PDGF and bFGF increased obviously in lung tissue of H group,whereas the expression of bFGF and PDGF of H+Li group reduced obviously but that of CGRP was not influenced.CONCLUSION:SCGRP reduction and expression increasion increasion of PDGF.bFGF have close relation with the formation of CHPH;Inhibiting the expression of PDGF.bFGF may be an aspect of the mechanism of preventing and treating HPH by ligustrazine.