A defined in vitro system for packaging of bacteriophage T3 DNA |
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| Authors: | K Hamada H Fujisawa T Minagawa |
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| Affiliation: | 1. Department of Mechanical Engineering, Ahmadu Bello University, Zaria, Nigeria;2. Department of Materials Science and Engineering, University of Ghana, Legon, Ghana;3. Department of Chemical Engineering, Ahmadu Bello University, Zaria, Nigeria;4. Institute of Applied Science and Technology, University of Ghana, Legon, Ghana |
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| Abstract: | ![]()
Using purified components, we have constructed an in vitro system for packaging of mature phage T3 DNA. In addition to mature T3 DNA, the system contained T3 proheads and the products of gene 18 (gp18) and gene 19 (gp19). The reaction required Mg2+, ATP, and polyvinyl alcohol. Spermidine was stimulatory but not absolutely required for the packaging reaction. Polyvinyl alcohol could be replaced by polyethylene glycol. The packaging efficiency decreased with decreasing molecular weight of the polymer, and low molecular weight polyols such as sucrose, sorbitol, and glycerol were inactive. The packaging reaction exhibited a sigmoidal relationship with respect to the concentration of ATP with the concentration for half maximal activity about 15 microM. A nonhydrolyzable ATP analog, adenosine 5'-O-(3-thiotriphosphate), inhibited the packaging reaction. |
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