| 人胎盘基因表达谱芯片的初步研究 |
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| 引用本文： | 朱利娜,马文丽,毛向明,李凌,冯春琼,郑文岭. 人胎盘基因表达谱芯片的初步研究[J]. 南方医科大学学报, 2002, 22(5): 400-402 |
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| 作者姓名： | 朱利娜 马文丽 毛向明 李凌 冯春琼 郑文岭 |
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| 作者单位： | 第一军医大学分子生物学研究所,全军生物芯片重点实验室,广东,广州,510515;广州军区广州总医院分子肿瘤学研究所,广东,广州,510010 |
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| 基金项目： | 国家自然科学基金(39880032),广州市重点科技攻关项目(990448022) |
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| 摘 要： | 目的 制备人胎盘基因表达谱芯片,前用于基因差异表达分析。方法用基因芯片点样仪将胎盘基因文库探针打印在氨基包被的玻片上制备表达谱芯片。分别提取对照组三氧化二砷处理的K562细胞总RNA,纯化mRNA后反转录成cDNA。再以限制性显示技术进行荧光标记,将两组荧光标记的样品混合后与芯片进行杂交。杂交后清洗芯片,干燥后对芯片进行扫描,分析杂交结果。结果 建立了较可靠的制备与检测基基表达芯片的方法,并筛选出45个差异表达基因片段。结论 制备的人胎盘基因表达谱芯片可有效地应用于基因的差异表达分析。
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| 关 键 词： | 基因芯片 基因表达谱 胎盘 |
| 文章编号： | 1000-2588(2002)05-0400-03 |
| 修稿时间： | 2001-12-05 |
Preliminary study of DNA microarray of human placenta gene expression profile |
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| ZHU Li-na,MA Wen-li,MAO Xiang-ming,LI Ling,FENG Chun-qiong,ZHENG Wen-lingInstitute of Molecular Biology and Key Laboratory for Biochip of PLA,First Military Medical University,Guangzhou,China. Preliminary study of DNA microarray of human placenta gene expression profile[J]. Journal of Southern Medical University, 2002, 22(5): 400-402 |
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| Authors: | ZHU Li-na MA Wen-li MAO Xiang-ming LI Ling FENG Chun-qiong ZHENG Wen-lingInstitute of Molecular Biology Key Laboratory for Biochip of PLA First Military Medical University Guangzhou China |
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| Affiliation: | ZHU Li-na,MA Wen-li,MAO Xiang-ming,LI Ling,FENG Chun-qiong,ZHENG Wen-lingInstitute of Molecular Biology and Key Laboratory for Biochip of PLA,First Military Medical University,Guangzhou510515,China, Institute of Molecular Oncology,GuangZhou Gener |
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| Abstract: | Objective To prepare human placenta genechip for analyzing differential gene expressions. Methods The targetgene amplified from Hybrid Hunter cDNA library was dotted onto the slides by the arrayer (PixSys 5500). Total RNA fromK562 cells treated with or without arsenic trioxide was cxtracted, the mRNA purified and cDNA synthesized. Fluorescentlabeling of the samples was performed by restriction display PCR, followed by hybridization with the microarray that wassubsequently washed and scanned, with the derived signals analyzed by computer. Results A reliable and specific method forpreparing and assessing gene expression profile microarray was established. with which a total of 45 differentially expressedgene fragments were isolated. Conclusion The genechips can be helpful in the analysis ofdifferential gene expressions. |
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| Keywords: | genechip gene expression profile placenta |
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