端粒酶蛋白催化亚单位基因及其蛋白在胃癌中的表达

目的　探讨端粒酶蛋白催化亚单位 (hTERT)基因mRNA及其蛋白在胃癌发生、发展中的重要作用以及在胃癌诊断中的意义。方法　应用原位杂交方法检测 5 3例胃癌标本中hTERTmRNA的表达 ,应用免疫组化S P法检测相同标本中hTERT蛋白的表达。结果　 5 3例胃癌标本中 ,hTERTmRNA阳性 41例 (77% ) ,其中低分化腺癌阳性 18例 (82 % ) ,高分化腺癌阳性 2 1例 (81% ) ,黏液腺癌及黏液细胞癌阳性 2例 (2 / 5 ) ;hTERT蛋白阳性共 46例 (87% ) ,其中低分化腺癌阳性 2 0例 (91% ) ,高分化腺癌阳性 2 5例(96 % ) ,黏液腺癌及黏液细胞癌阳性 1例 (1/ 5 ) ;hTERTmR NA及hTERT蛋白的表达在低分化腺癌组与高分化腺癌组之间差异未见显著性 (P >0 0 5 ) ,但hTERTmRNA与hTERT蛋白之间呈正相关 (rS=0 6 2 3,P <0 0 1)。结论　hTERTmRNA及蛋白表达的检测有可能为胃癌的诊断及鉴别诊断提供一种新的形态学的指标

Expression of human telomerase catalytic subunit gene and its protein in gastric cancer

Objective To investigate the expression of human telomerase catalytic subunit gene and the correlation of its mRNA and protein in gastric cancer. Methods Expression of hTERT mRNA was detected by in situ hybridization(ISH), and expression of hTERT protein was detected by S-P immunohistochemical technique in 53 patients with gastric carcinoma. Results Positive cases of hTERT mRNA were detected in 41(77%) patients with gastric carcinoma, in which 18(82%) were poor differentiated adenocarcinoma, 21(81%) well differentiated adenocarcinoma and 2 mucoid carcinoma(2/5). Positive dying of hTERT protein was detected in 46(87%) patients with gastric carcinoma, in which 20(91%) were poor differentiated adenocarcinoma, 25(96%) well differentiated adenocarcinoma and 1 mucoid carcinoma. Expression of hTERTmRA between poor differentiated adenocarcinoma and well differentiated adenocarcinoma was not significanty different, so was hTERT protein(P>0.05). Expression of hTERT mRNA and hTRET protein showed significant positive correlation(r s=0.623, P<0.001). Conclusion The dectetion of hTERT gene expression may provide a new morphological method for diagnosis and distinctive diagnosis of gastric cancer.