结肠癌血清IGF-1水平及肿瘤组织中VEGF-C、VEGFR-3表达与淋巴结转移的相关性研究

目的探讨结肠癌血清胰岛素生长因子1(IGF-1)水平及肿瘤组织中血管内皮生长因子-C(VEGF-C)、血管内皮生长因子受体3(VEGFR-3)表达与微淋巴管生成以及淋巴结转移的相关性。方法收集60例结肠癌和60例结肠腺瘤患者血清样本和组织标本,分为结肠癌组和结肠腺瘤组,同一结肠癌组织标本癌旁正常组织作为癌旁正常组。另选取60例体检健康者作为健康组,健康组收集血清作为对照。比较各组血清IGF-1水平,组织中VEGF-C、VEGFR-3表达水平以及微淋巴管密度(LMVD)值,并分析其与结肠癌病理特征关系以及相互间关系。结果结肠癌组血清IGF-1水平显著高于结肠腺瘤组和健康组;结肠癌组中VEGF-C、VEGFR-3表达水平以及LMVD值均显著高于结肠腺瘤组和癌旁正常组,差异有统计学意义(P均<0.05);血清IGF-1水平,组织中VEGF-C、VEGFR-3表达水平以及LMVD值均与浸润程度和淋巴结转移有关,差异有统计学意义(P均<0.05),与性别、年龄无关,差异无统计学意义(P>0.05);IGF-1与VEGF-C、VEGFR-3及LMVD均呈正相关(r=0.68,r=0.66,r=0.55,P<0.05),VEGF-C和VEGFR-3与LMVD均呈正相关(r=0.60,r=0.62,P<0.05)。结论结肠癌血清IGF-1水平与组织VEGF-C、VEGFR-3阳性表达均明显上调,共同参与微淋巴管生成以及淋巴结转移,促进结肠癌发生发展。

Correlation between serum IGF-1 level in colon cancer,expression levels of VEGF-C and VEGFR-3 in tumor tissues and lymph node metastasis

Objective To explore the correlation between the level of serum insulin growth factor1(IGF-1)in colon cancer,the expression levels of vascular endothelial growth factor-C(VEGF-C)and vascular endothelial growth factor receptor 3(VEGFR-3)in tumor tissues and lymphangiogenesis and lymph node metastasis. Methods Serum samples and tissues specimens were collected from 60 patients with colon cancer and 60 patients with colon adenoma,and they were divided into colon cancer group and colon adenoma group. And the normal adjacent tissues of the same colon cancer tissue specimens were served as normal adjacent group. Another 60 healthy examiners were enrolled as healthy group,and the serum was collected as control in healthy group. The serum IGF-1 level,expression levels of VEGF-C and VEGFR-3 and lymphatic microvessel density(LMVD)value were compared among the groups. And their relationship with pathological features of colon cancer and their interrelation were analyzed. Results The serum IGF-1 level in colon cancer group was significantly higher than that in colon adenoma group and healthy group(P<0.05). The expression levels of VEGF-C and VEGFR-3 and LMVD value in colon cancer group were significantly higher than those in colon adenoma group and normal adjacent group(all P<0.05). The serum IGF-1 level,expression levels of VEGF-C and VEGFR-3 in tissues and LMVD value were related to the invasion degree and lymph node metastasis(all P<0.05),but were not related to gender and age(P>0.05). IGF-1 was positively correlated with VEGF-C,VEGFR-3 and LMVD(r=0.68,r=0.66,r=0.55,P<0.05),and VEGF-C and VEGFR-3 were positively correlated with LMVD(r=0.60,r=0.62,P<0.05). Conclusions Serum IGF-1 level in colon cancer and positive expression levels of VEGF-C and VEGFR-3 in tissues are significantly up-regulated,and they jointly participate in lymphangiogenesis and lymph node metastasis,and promote the occurrence and development of colon cancer.