负压吸引对兔视网膜组织结构的影响

目的 探讨准分子激光原位角膜磨镶术(LASIK)中负压吸引对兔视网膜结构的影响。方法 32只健康新西兰大耳白兔随机分为正常对照组、实验对照组、负压吸引45s组和吸引3min组。实验对照组只进行激光治疗，负压吸引各组先用负压发生器吸引眼角巩膜缘，持续45s及3min，分别于术后即刻、7d、14d、28d在光镜和电镜下观察兔眼视神经和视网膜组织结构的变化，并与对照组进行比较。结果 与正常对照组相比，实验对照组视神经和视网膜结构无改变。负压吸引45s组各时间点视神经和视网膜细胞改变轻微；3min组术后即刻～14d神经纤维和视网膜视细胞结构明显异常，至术后28d时恢复正常。结论 LASIK术中负压吸引引起的眼压急剧升高，可能会导致视网膜和视神经细胞的亚细胞结构改变，但这种改变为可逆的;负压吸引持续时间越长改变越明显。

Effect of negative pressure suction on rabbit retina structure

ObjectiveTo study the effect of negative pressure suction during laser in situ keratomileusis (LASIK) on rabbit retinal structure.Methods32 New Zealand white rabbits were randomly divided into normal control, experimental control, negative suction 45 seconds and negative suction 3 minutes groups. Experimental control groups were treated only using eximer laser. In negative suction groups, rabbit eyes were suction for 45 seconds or 3 minutes by negative pressure generator. The tissues of optic nerve and retina both after negative pressure suction and of normal rabbit eyes were examined with electron and light microscope 0, 7, 14, 28 days after operation. ResultsThere was no change in the tissues of optic nerve and retina between normal control and experimental control. After the negative pressure suction for 45 s, the optic nerve and retinal cells changed lightly at different time point. After the negative pressure suction for 3 min, the optic nerve fibers and retinal cells changed sharply during postoperative instant to 14th days, which can be recovered until 28 days after operation.ConclusionUltrastructural changes of retina and optic nerve may be induced by acute intraocular pressure elevation caused by negative pressure suction during LASIK.