| 大血藤对破骨细胞活性及成骨细胞增殖分化作用的研究 |
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| 引用本文： | 陈丽珍,周英,黄俊飞,张雪,俸婷婷.大血藤对破骨细胞活性及成骨细胞增殖分化作用的研究[J].中国中药杂志,2015,40(22):4463-4468. |
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| 作者姓名： | 陈丽珍 周英 黄俊飞 张雪 俸婷婷 |
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| 作者单位： | 贵州大学药学院, 贵州贵阳 550025;贵州省中药民族药创制工程中心, 贵州贵阳 550025;贵州省药食同源植物资源研究开发中心, 贵州贵阳 550025,贵州大学药学院, 贵州贵阳 550025;贵州省中药民族药创制工程中心, 贵州贵阳 550025;贵州省药食同源植物资源研究开发中心, 贵州贵阳 550025,贵州大学药学院, 贵州贵阳 550025;贵州省中药民族药创制工程中心, 贵州贵阳 550025;贵州省药食同源植物资源研究开发中心, 贵州贵阳 550025,贵州大学药学院, 贵州贵阳 550025;贵州省中药民族药创制工程中心, 贵州贵阳 550025;贵州省药食同源植物资源研究开发中心, 贵州贵阳 550025,贵州大学药学院, 贵州贵阳 550025;贵州省中药民族药创制工程中心, 贵州贵阳 550025;贵州省药食同源植物资源研究开发中心, 贵州贵阳 550025 |
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| 基金项目： | 贵阳市科技计划项目(筑科合同[2012204]9号);贵州省国际科技合作计划项目(黔科合外G字[2013]7009号);贵州大学引进人才科研项目[贵大人基合字(2012)010号];贵州省科学技术基金项目(黔科合J字[2012]2173号);贵州省药食同源植物资源研究开发中心项目(黔科合G字[2014]4003号);贵州省高校工程技术研究中心建设项目[黔教合KY字(2012)018号];贵州大学研究生创新基金项目(研农2015020) |
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| 摘 要： | 该研究采用1α, 25-(OH)2VitD3诱导兔骨髓细胞法获得破骨细胞,以形态学观察、HE染色、抗酒石酸酸性磷酸酶(TRAP)染色和骨吸收陷窝甲苯胺蓝染色来鉴定破骨细胞,用TRAP+细胞计数、TRAP活力测定和骨吸收陷窝的数量及面积来检测大血藤对破骨细胞分化及骨吸收功能的影响。培养MC3T3-E1Subclone 14(小鼠颅顶前骨细胞亚克隆14)细胞,用MTT法、碱性磷酸酶活力测定检测大血藤对其增殖和分化的影响。结果表明大血藤醇提物和水煎物低、中、高剂量组(2,20,200 mg·L-1)对破骨细胞分化及骨吸收功能具有抑制作用,并具有剂量依赖性。大血藤醇提物和水煎物低、中剂量组具有促进MC3T3-E1Subclone 14细胞增殖分化的作用,说明大血藤对骨质疏松有一定的防治作用,这种作用可能是通过抑制破骨细胞活性和促进成骨细胞增殖分化来实现的。
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| 关 键 词： | 大血藤 破骨细胞 成骨细胞 骨吸收 增殖分化 |
| 收稿时间： | 3/9/2015 12:00:00 AM |
Research on effect of Sargentodoxae caulis on activity of osteoclasts and proliferation differentiation of osteoblasts |
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| CHEN Li-zhen,ZHOU Ying,HUANG Jun-fei,ZHANG Xue and FENG Ting-ting.Research on effect of Sargentodoxae caulis on activity of osteoclasts and proliferation differentiation of osteoblasts[J].China Journal of Chinese Materia Medica,2015,40(22):4463-4468. |
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| Authors: | CHEN Li-zhen ZHOU Ying HUANG Jun-fei ZHANG Xue and FENG Ting-ting |
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| Institution: | School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;Guizhou Engineering Center for Innovative Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China;Research and Development Center for Medicinal and Edible Plant Resources of Guizhou Province, Guiyang 550025, China,School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;Guizhou Engineering Center for Innovative Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China;Research and Development Center for Medicinal and Edible Plant Resources of Guizhou Province, Guiyang 550025, China,School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;Guizhou Engineering Center for Innovative Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China;Research and Development Center for Medicinal and Edible Plant Resources of Guizhou Province, Guiyang 550025, China,School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;Guizhou Engineering Center for Innovative Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China;Research and Development Center for Medicinal and Edible Plant Resources of Guizhou Province, Guiyang 550025, China and School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;Guizhou Engineering Center for Innovative Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China;Research and Development Center for Medicinal and Edible Plant Resources of Guizhou Province, Guiyang 550025, China |
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| Abstract: | |
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| Keywords: | Sargentodoxae caulis osteoclasts osteoblasts bone resorption proliferation and differentiation |
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