Screening for Human Parvovirus B19 Infection in Egyptian Family Replacement Blood Donors |
| |
| Authors: | Rabab Hasanain Ahmed Hasanain Rania M. Saleh Fadia M. Attia Hanaa H. Gomaa |
| |
| Affiliation: | 1.Department of Botany, Faculty of Science, Suez Canal University, Ismailia, Egypt ;2.Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt |
| |
| Abstract: | Up till now, screening for human parvovirus B19 is not routine in national Egyptian blood bank strategy. Blood samples were collected from 500 healthy blood donors within the age range from 18 to 45 years old attending the blood bank of Suez Canal University Hospital, Ismailia, Egypt. Sera were separated and stored at − 20 °C. Serum samples were screened for anti-human parvovirus B19 IgM and IgG antibodies and B19 genome using ELISA and real-time PCR respectively. Frequency of B19 IgM and B19 IgG antibodies was 6.20%, and 80.20% respectively, and the prevalence of B19 genome was 3.00%. There is a high frequency of human parvovirus B19 among Egyptian blood donors; therefore, serological screening for B19 is warranted. |
| |
| Keywords: | Human parvovirus B19 Blood transfusion ELISA Real-time PCR |
|
|
