抗细胞膜DNA抗体在三种自身抗体阴性系统性红斑狼疮中的诊断价值

目的 研究抗细胞膜DNA（mDNA）抗体在抗Sm抗体、抗双链DNA（dsDNA）抗体和抗核小体抗体（AnuA）等特异性自身抗体阴性的系统性红斑狼疮（SLE）中的诊断意义。方法 随机选择145例SLE患者。以Raji细胞为底物，采用间接免疫荧光法检测SLE患者血清抗mDNA抗体和抗dsDNA抗体，采用免疫印迹法检测抗Sm抗体，酶联免疫吸附法测定AnuA。分析抗mDNA抗体在其他特异性自身抗体阴性SLE患者中的诊断价值。结果 抗mDNA抗体在SLE中的阳性率为69．7％，明显高于抗Sm抗体（19．7％）、抗dsDNA抗体（31．9％）和AnuA（45．8％，均P〈0．01）。在抗dsDNA抗体阴性的SLE患者中，抗mDNA抗体的阳性率达64．3％；在抗Sm抗体阴性的SLE患者中，抗mDNA抗体的阳性率为70．2％；而AnuA阴性的SLE患者中，抗mDNA抗体的阳性率为60．3％。结论 抗mDNA抗体是一种敏感性较高的SLE血清学标志之一，尤其对抗Sm抗体、抗dsDNA抗体或AnuA阴性SLE的诊断具有重要参考意义。

Significance of anti-cell membrane associated DNA antibodies in diagnosis of systemic lupus erythematosus lacking of specific autoantibodies

Objective To investigate the effects of anti-cell membrane associated DNA (mDNA) antibodies in the diagnosis of systemic lupus erythematosus (SLE) lacking of specific autoantibodies including anti Sm,anti ds-DNA,and anti-nucleosome antibodies.Methods Indirect immunofluorescence assay was used to measure anti-mDNA antibodies in serum of 145 SLE patients,and indirect immunofluorescence,Western-blot and ELISA were used to detect the anti-dsDNA ,anti-Sm and anti- nueleosome antibodies respectively to analysis the value of anti-mDNA antibodies on the specific autoantibodies negative patients with SLE.Results The sensitivity for anti-mDNA antibodies (69.7%) in SLE was significantly higher than anti-Sm (19.7%),anti-dsDNA ( 31.9% ) and anti-nucleosome (45.8% ).The incidences of anti-mDNA antibodies in SLE lacking of anti-dsDNA,Sm and anti-nueleosome antibodies (AnuA) were 64.3% ,70.2% and 60.3% respectively.Conclusion Anti-mDNA antibodies are serologic marker of SLE and important in diagnosis of SLE lacking of anti-dsDNA,Sm and nucleosome antibodies.