| hnRNPB1基因的RNA干扰抑制肺癌细胞A549增殖研究 |
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| 引用本文： | 蒲丹,李为民,周陶友,吴迪,陈小兵,陈敏,唐凤鸣,韩娟. hnRNPB1基因的RNA干扰抑制肺癌细胞A549增殖研究[J]. 四川大学学报(医学版), 2009, 40(3) |
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| 作者姓名： | 蒲丹 李为民 周陶友 吴迪 陈小兵 陈敏 唐凤鸣 韩娟 |
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| 作者单位： | 四川大学华西医院,呼吸内科,成都,610041;四川大学华西医院,呼吸内科,成都,610041;四川大学华西医院,生物治疗国家重点实验室 |
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| 基金项目： | 国家自然科学基金,教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的 探讨特异性小分子干扰(small interference,siRNA)抑制肺癌A549细胞核内不均一核糖蛋白B1(heterogeneous nuclear ribonucleoprotein,hnRNPB1)基因的表达后,对A549细胞增殖的影响. 方法构建hnRNPB1特异性的siRNA真核细胞表达载体并转染人肺癌细胞株A549,观察重组载体分别在第1、4及6周对A549细胞hnRNPB1基因的干扰效果以及对肺癌细胞周期及凋亡的影响.结果 特异性siRNA真核表达可显著抑制肺癌细胞hnRNPB1基因的表达,使hnRNPB1 mRNA的表达减少46%～73%,蛋白表达减少77.69%～83.04%,作用时间至少可维持克隆形成后4周,同时,hnRNPB1基因表达下调抑制了A549细胞在体外的增殖,促进了肺癌细胞的凋亡.结论 特异性siRNA能特异、高效地抑制肺癌细胞株A549细胞hnRNPB1基因的表达,RNAi可能为肺癌的基因治疗提供新策略.
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| 关 键 词： | 肺癌 核内不均一核糖核蛋白 B1 小分子干扰 RNA |
The Inhibitory Effects of siRNA Expression Vector on Cell Proliferation and Expression of hnRNPB_1 Gene in Lung Cancer A549 Cells |
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| PU Dan,LI Wei-min,ZHOU Tao-you,WU Di,CHEN Xiao-bing,CHEN Min,TANG Feng-ming,HAN Juan. The Inhibitory Effects of siRNA Expression Vector on Cell Proliferation and Expression of hnRNPB_1 Gene in Lung Cancer A549 Cells[J]. Journal of Sichuan University. Medical science edition, 2009, 40(3) |
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| Authors: | PU Dan LI Wei-min ZHOU Tao-you WU Di CHEN Xiao-bing CHEN Min TANG Feng-ming HAN Juan |
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| Affiliation: | PU Dan,LI Wei-min,ZHOU Tao-you,WU Di,CHEN Xiao-bing,CHEN Min,TANG Feng-ming,HAN Juan.Department of Respiratory Diseases,West China Hospital,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective To investigate the inhibitory effects of specific siRNA on the expression of heterogeneous nuclear ribonucleoproteins(hnRNPB1)and cell proliferation in lung cancer A549 cells.MethodsAfter the construction of siRNA expression vector for hnRNPB1,cultured A549 cells were tansfected with the specific siRNA vector,so RNA of A549 cells was interfered by RNA interference technique.The cells were retrieved at 1st,4th and 6th week after transfection,cell cycles and cell apoptosis were measured with flow cy... |
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| Keywords: | B1 RNA |
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